Detail publikace
Use of Calcium Chloride for Removal of Polysaccharides During DNA Isolation from Tropical Fruit Products
FIALOVÁ, L. ŽYLKOVÁ, K. MÁROVÁ, I.
Originální název
Use of Calcium Chloride for Removal of Polysaccharides During DNA Isolation from Tropical Fruit Products
Typ
abstrakt
Jazyk
angličtina
Originální abstrakt
Food products made out of certain fruits (such as mango) may be subject to adulteration. The adulteration may be detected by various methods, including PCR-based ones. However, PCR-based methods are susceptible to inhibition if the DNA sample is contaminated with certain polysaccharides. Furthermore, polysaccharide contamination is notoriously difficult to remove. In this work we demonstrate the use of calcium chloride for successful removal of potentially inhibitory polysaccharides from samples which are known to contain them (mango and banana puree). We also address the potential inhibitory effect of calcium chloride itself and we show a way in which polysaccharide precipitation by calcium chloride may be added to a DNA isolation protocol without hampering the amplifiability of the isolated DNA.
Klíčová slova
PCR, DNA isolation, polysaccharides, calcium chloride, mango
Autoři
FIALOVÁ, L.; ŽYLKOVÁ, K.; MÁROVÁ, I.
Vydáno
23. 10. 2023
Nakladatel
Institute of Analytical Chemistry of the CAS, v. v. i., Veveri 97, 602 00 Brno, Czech Republic
Místo
Brno
ISBN
978-80-908154-0-7
Kniha
CECE 2023 18th International Interdisciplinary Meeting on Bioanalysis
Edice
1
Číslo edice
1
Strany od
50
Strany do
50
Strany počet
1
URL
BibTex
@misc{BUT185429,
author="Lenka {Fialová} and Kateřina {Žylková} and Ivana {Márová}",
title="Use of Calcium Chloride for Removal of Polysaccharides During DNA Isolation from
Tropical Fruit Products",
booktitle="CECE 2023 18th International Interdisciplinary Meeting on Bioanalysis",
year="2023",
series="1",
edition="1",
pages="50--50",
publisher="Institute of Analytical Chemistry of the CAS, v. v. i., Veveri 97, 602 00 Brno, Czech Republic",
address="Brno",
isbn="978-80-908154-0-7",
url="https://www.ce-ce.org/user_uploads/CECE_2023/CECE%202023%20Proceedings.pdf",
note="abstract"
}