Detail publikačního výsledku
Morphological Study of PHA Producing Bacteria
HRUBANOVÁ, K.; SIKOROVÁ, P.; MRÁZOVÁ, K.; NEBESÁŘOVÁ, J.; OBRUČA, S.; KRZYŽÁNEK, V.
Originální název
Morphological Study of PHA Producing Bacteria
Anglický název
Morphological Study of PHA Producing Bacteria
Druh
Stať ve sborníku v databázi WoS či Scopus
Originální abstrakt
Our comprehensive morphological study indicates that the method involving cryo-SEM imaging in combination with highpressure HPF freezing and perpendicular freeze-fracturing, is an excellent technique for analysing highly hydrated samples. Cells containing polymer particles, which are elastic even at very low temperatures, were visualized using this method. By TEM it is able to monitor the distribution of PHB granules inside the microbial cells. SBFSEM provides spatial information. A comparison of the morphology of the internal and external cell structure shows that the Cupriavidus necator H16 contains significant amounts of PHA granules, which are distributed throughout the whole cell volume .
Anglický abstrakt
Our comprehensive morphological study indicates that the method involving cryo-SEM imaging in combination with highpressure HPF freezing and perpendicular freeze-fracturing, is an excellent technique for analysing highly hydrated samples. Cells containing polymer particles, which are elastic even at very low temperatures, were visualized using this method. By TEM it is able to monitor the distribution of PHB granules inside the microbial cells. SBFSEM provides spatial information. A comparison of the morphology of the internal and external cell structure shows that the Cupriavidus necator H16 contains significant amounts of PHA granules, which are distributed throughout the whole cell volume .
Klíčová slova
polyhydroxyalkanoates; electron microscopy; Cupriavidus necator
Klíčová slova v angličtině
polyhydroxyalkanoates; electron microscopy; Cupriavidus necator
Autoři
HRUBANOVÁ, K.; SIKOROVÁ, P.; MRÁZOVÁ, K.; NEBESÁŘOVÁ, J.; OBRUČA, S.; KRZYŽÁNEK, V.
Rok RIV
2024
Vydáno
31.10.2023
Nakladatel
Oxford Academic
Místo
Oxford
Kniha
Mictoscopy and Microanalysis
ISSN
1435-8115
Periodikum
MICROSCOPY AND MICROANALYSIS
Svazek
29
Číslo
1
Stát
Spojené státy americké
Strany od
883
Strany do
884
Strany počet
2
URL
Plný text v Digitální knihovně
BibTex
@inproceedings{BUT187572,
author="Kamila {Hrubanová} and Pavlína {Sikorová} and Kateřina {Mrázová} and Jana {Nebesářová} and Stanislav {Obruča} and Vladislav {Krzyžánek}",
title="Morphological Study of PHA Producing Bacteria",
booktitle="Mictoscopy and Microanalysis",
year="2023",
journal="MICROSCOPY AND MICROANALYSIS",
volume="29",
number="1",
pages="883--884",
publisher="Oxford Academic",
address="Oxford",
doi="10.1093/micmic/ozad067.436",
issn="1431-9276",
url="https://watermark.silverchair.com/ozad067.436.pdf?token=AQECAHi208BE49Ooan9kkhW_Ercy7Dm3ZL_9Cf3qfKAc485ysgAAA24wggNqBgkqhkiG9w0BBwagggNbMIIDVwIBADCCA1AGCSqGSIb3DQEHATAeBglghkgBZQMEAS4wEQQM6zvKSZpfNQkegG0tAgEQgIIDIdKmjtZo1JTIbtb6RFZkFuVEwyCH50hW3BGT1LvIQEWQLtR2vrZuSYNt0-RFn4X-My459ZXHgDgT3rXVyN2hr5IheLy7h3coAB9uWNHZyMpECgDPA-kncAOzVulTsU3Kjj0NO5WEeWIPn_AD_PzvxEI3tKdoFX0tkT_Mig2zipqdYSafxFl9M7Eo3l-paA0fN24q4yb7KIe_uNS4DIL9MK9qy-r74U0ObIq4W00RuFrCimenl2YzSQ9e8U42dFsaNmZ9C7mod63Zd98fCNbOydwTwq9_puW6rJVG2OY7jDWF"
}