Detail publikačního výsledku
Self-entrapment of Azotobacter vinelandii cultures by gelation of their exopolysaccharides: A way towards next-generation bioinoculants
ČERNAYOVÁ, D.; SÚKENÍK, M.; OBRUČA, S.; SMILEK, J.; KALINA, M.; MRÁZOVÁ, K.; HRUBANOVÁ, K.; KRZYŽÁNEK, V.; SEDLÁČEK, P.
Originální název
Self-entrapment of Azotobacter vinelandii cultures by gelation of their exopolysaccharides: A way towards next-generation bioinoculants
Anglický název
Self-entrapment of Azotobacter vinelandii cultures by gelation of their exopolysaccharides: A way towards next-generation bioinoculants
Druh
Článek WoS
Originální abstrakt
Encapsulation of Plant Growth-Promoting Rhizobacteria (PGPR) in hydrogel carriers is a cutting-edge approach in developing agricultural bioinoculants, aiming to improve soil fertility and crop yield. Hydrogels provide protection against environmental stress, though traditional methods using external gel-forming agents limit economic viability. This study presents a novel approach, demonstrating the entrapment of Azotobacter vinelandii, a promising PGPR, within a gel matrix formed by Ca2+-induced crosslinking of its own exopolysaccharide, alginate. Among the five strains evaluated, A. vinelandii DSM 87, DSM 720, and DSM 13529 showed the highest alginate production, peaking at 4.9 +/- 0.6 g/L, 3.5 +/- 0.5 g/L, 3.8 +/- 0.8 g/L, and enabling stable gel formation by the fourth day of cultivation. These strains also exhibited molecular weights and chemical structures of alginate suitable for effective gelation upon Ca2+addition. Additionally, these strains demonstrated significant plant growth-promoting activities, including indole acetic acid production (up to 10.5 mu g/mL), siderophore release, and phosphate solubilization, further validating their potential for sustainable bioinoculant production. Finally, the viability of the A.vinelandii cells released from the gels was experimentally verified. Our findings support a feasible, cost-effective method for bioinoculant production that leverages A. vinelandii's intrinsic capabilities, offering a sustainable alternative to conventional agricultural practices.
Anglický abstrakt
Encapsulation of Plant Growth-Promoting Rhizobacteria (PGPR) in hydrogel carriers is a cutting-edge approach in developing agricultural bioinoculants, aiming to improve soil fertility and crop yield. Hydrogels provide protection against environmental stress, though traditional methods using external gel-forming agents limit economic viability. This study presents a novel approach, demonstrating the entrapment of Azotobacter vinelandii, a promising PGPR, within a gel matrix formed by Ca2+-induced crosslinking of its own exopolysaccharide, alginate. Among the five strains evaluated, A. vinelandii DSM 87, DSM 720, and DSM 13529 showed the highest alginate production, peaking at 4.9 +/- 0.6 g/L, 3.5 +/- 0.5 g/L, 3.8 +/- 0.8 g/L, and enabling stable gel formation by the fourth day of cultivation. These strains also exhibited molecular weights and chemical structures of alginate suitable for effective gelation upon Ca2+addition. Additionally, these strains demonstrated significant plant growth-promoting activities, including indole acetic acid production (up to 10.5 mu g/mL), siderophore release, and phosphate solubilization, further validating their potential for sustainable bioinoculant production. Finally, the viability of the A.vinelandii cells released from the gels was experimentally verified. Our findings support a feasible, cost-effective method for bioinoculant production that leverages A. vinelandii's intrinsic capabilities, offering a sustainable alternative to conventional agricultural practices.
Klíčová slova
Azotobacter vinelandii; Alginate; Polyhydroxyalkanoates; Hydrogels; Bioinoculants
Klíčová slova v angličtině
Azotobacter vinelandii; Alginate; Polyhydroxyalkanoates; Hydrogels; Bioinoculants
Autoři
ČERNAYOVÁ, D.; SÚKENÍK, M.; OBRUČA, S.; SMILEK, J.; KALINA, M.; MRÁZOVÁ, K.; HRUBANOVÁ, K.; KRZYŽÁNEK, V.; SEDLÁČEK, P.
Vydáno
17.04.2025
Nakladatel
ELSEVIER SCI LTD
Místo
London
ISSN
0144-8617
Periodikum
Carbohydrate Polymers
Svazek
360
Číslo
C
Stát
Spojené království Velké Británie a Severního Irska
Strany od
1
Strany do
11
Strany počet
11
URL
Plný text v Digitální knihovně
BibTex
@article{BUT197917,
author="Diana {Černayová} and Martin {Súkeník} and Stanislav {Obruča} and Jiří {Smilek} and Michal {Kalina} and Kateřina {Mrázová} and Kamila {Hrubanová} and Vladislav {Krzyžánek} and Petr {Sedláček}",
title="Self-entrapment of Azotobacter vinelandii cultures by gelation of their exopolysaccharides: A way towards next-generation bioinoculants",
journal="Carbohydrate Polymers",
year="2025",
volume="360",
number="C",
pages="1--11",
doi="10.1016/j.carbpol.2025.123607",
issn="0144-8617",
url="https://www.sciencedirect.com/science/article/pii/S0144861725003881"
}