Fate of Enrofloxacin and Its Metabolites in Chicken Faeces after Antibiotic Treatment

Fate of Enrofloxacin and Its Metabolites in Chicken Faeces after Antibiotic Treatment

Konferenční sborník (ne stať)

This study investigates the occurrence, persistence, and degradation behaviour of the antibiotic enrofloxacin (ENR) and its microbiologically active metabolites in fecal samples from chickens reared at a commercial farm in the Czech Republic. An optimised ultrasound-assisted extraction method followed by UHPLC-ESI-TQ was used for the quantification of ENR and its metabolites. ENR was detected in all samples at concentrations reaching tens of mg/kg, while ciprofloxacin (CIP) and another identified metabolite and degradation product were also consistently present. Both ENR and CIP concentrations decreased exponentially over time, following first-order elimination and degradation kinetics. Despite this decline, ENR residues persisted even one month after the treatment. The results demonstrate that poultry manure contains significant levels of active antibiotic residues and metabolites, highlighting the environmental risk associated with of applying improperly treated chicken excrement to fields as fertiliser and its potential contribution to the spread of antibiotic resistance.

This study investigates the occurrence, persistence, and degradation behaviour of the antibiotic enrofloxacin (ENR) and its microbiologically active metabolites in fecal samples from chickens reared at a commercial farm in the Czech Republic. An optimised ultrasound-assisted extraction method followed by UHPLC-ESI-TQ was used for the quantification of ENR and its metabolites. ENR was detected in all samples at concentrations reaching tens of mg/kg, while ciprofloxacin (CIP) and another identified metabolite and degradation product were also consistently present. Both ENR and CIP concentrations decreased exponentially over time, following first-order elimination and degradation kinetics. Despite this decline, ENR residues persisted even one month after the treatment. The results demonstrate that poultry manure contains significant levels of active antibiotic residues and metabolites, highlighting the environmental risk associated with of applying improperly treated chicken excrement to fields as fertiliser and its potential contribution to the spread of antibiotic resistance.

enrofloxacin; ciprofloxacin; metabolites; poultry; UHPLC-ESI-TQ; mass spectrometry, antibiotic residues; degradation kinetics; livestock farming; antibiotic resistance

enrofloxacin; ciprofloxacin; metabolites; poultry; UHPLC-ESI-TQ; mass spectrometry, antibiotic residues; degradation kinetics; livestock farming; antibiotic resistance

HAMPLOVÁ, M.; PARTOVI NASR, M.; ČEJKOVÁ, D.; FARKOVÁ, V.; ZLÁMALOVÁ GARGOŠOVÁ, H.

26.11.2025

Fakulta chemickej a potravinárskej technológie

Bratislava

978-80-8208-162-9

2

https://doi.org/10.5281/zenodo.17647142

Hamplova_M_2025