Antioxidant-Active Bacterial Cellulose via In Situ Phenolic Modification

Antioxidant-Active Bacterial Cellulose via In Situ Phenolic Modification

Abstrakt

Bacterial cellulose (BC) is a pure nanostructured polysaccharide that is produced predominantly by acetic acid bacteria of the genus Komagataeibacter through aerobic fermentation. BC differs from plant cellulose not only in the absence of lignin and hemicellulose, but also in its specific mechanical and physical properties. These include exceptional tensile strength, high flexibility, the ability to retain large amounts of water, and excellent biocompatibility. Its unique properties make BC an attractive biomaterial for biomedical, pharmaceutical, food, and cosmetic applications [1, 2]. BC is most commonly produced in carbohydrate- and nitrogen-containing media such as Hestrin–Schramm medium or media based on food-industry by-products. The composition of the culture medium and fermentation conditions can significantly influence the final structure and functionality of BC. This work focuses on bacterial cellulose as a carrier of bioactive compounds with antioxidant activity. We investigated in situ modification of BC during its biosynthesis using catechin and caffeic acid at concentrations of 0.1–1.0 mg/ml. The obtained BC-based composites were characterized for their antioxidant properties. Depending on the concentration of the incorporated phenolic compounds, the antioxidant activity increased in the range of [169.17–338.31 mgTE/g] compared to neat BC, while the content of bound polyphenols in the BC matrix reached [0.014–0.044 mgGAE/mg]. The results confirm that in situ functionalization of bacterial cellulose with catechin or caffeic acid is a suitable method for obtaining BC-based materials with high antioxidant activity without negatively affecting BC production. It was shown that in situ functionalization of bacterial cellulose with catechin or caffeic acid enables the production of BC with significant antioxidant activity while maintaining yield and quality.

Bacterial cellulose (BC) is a pure nanostructured polysaccharide that is produced predominantly by acetic acid bacteria of the genus Komagataeibacter through aerobic fermentation. BC differs from plant cellulose not only in the absence of lignin and hemicellulose, but also in its specific mechanical and physical properties. These include exceptional tensile strength, high flexibility, the ability to retain large amounts of water, and excellent biocompatibility. Its unique properties make BC an attractive biomaterial for biomedical, pharmaceutical, food, and cosmetic applications [1, 2]. BC is most commonly produced in carbohydrate- and nitrogen-containing media such as Hestrin–Schramm medium or media based on food-industry by-products. The composition of the culture medium and fermentation conditions can significantly influence the final structure and functionality of BC. This work focuses on bacterial cellulose as a carrier of bioactive compounds with antioxidant activity. We investigated in situ modification of BC during its biosynthesis using catechin and caffeic acid at concentrations of 0.1–1.0 mg/ml. The obtained BC-based composites were characterized for their antioxidant properties. Depending on the concentration of the incorporated phenolic compounds, the antioxidant activity increased in the range of [169.17–338.31 mgTE/g] compared to neat BC, while the content of bound polyphenols in the BC matrix reached [0.014–0.044 mgGAE/mg]. The results confirm that in situ functionalization of bacterial cellulose with catechin or caffeic acid is a suitable method for obtaining BC-based materials with high antioxidant activity without negatively affecting BC production. It was shown that in situ functionalization of bacterial cellulose with catechin or caffeic acid enables the production of BC with significant antioxidant activity while maintaining yield and quality.

bacterial cellulose, caffeic acid, catechin, antioxidant activity

bacterial cellulose, caffeic acid, catechin, antioxidant activity

27.11.2025

Vysoké učení technické v Brně

Vysoké učení technické v Brně, Fakulta chemická, Purkyňova 464/118, 612 00 Brno

978-80-214-6388-2

Studentská odborná konference Chemie je život 2025 Sborník abstraktů

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https://www.fch.vut.cz/vav/konference/sok/vystupy/sok-2025-sbornikabstraktu-pdf-p321225