Detail publikačního výsledku

Click Me If You Can: Wild-Type Aneurinibacillus Bacteria as Unlikely Architects of Functional Pha Copolymers

ŘEHÁKOVÁ, V.; BUCHTÍKOVÁ, I.; SEDLÁČEK, P.; KALINA, M.; TUŠLOVÁ, K.; OBRUČA, S.

Originální název

Click Me If You Can: Wild-Type Aneurinibacillus Bacteria as Unlikely Architects of Functional Pha Copolymers

Anglický název

Click Me If You Can: Wild-Type Aneurinibacillus Bacteria as Unlikely Architects of Functional Pha Copolymers

Druh

Abstrakt

Originální abstrakt

This study investigated wild-type Aneurinibacillus spp. as natural producers of diverse and functionally rich PHA copolymers without any genetic engineering. When grown on glucose with various lactones and diols, the strains incorporated an unusually wide range of monomers, demonstrating high substrate flexibility of their native PHA synthases. Using α-methyl-γ-butyrolactone, the researchers produced a rare α-branched PHA copolymer, confirmed by GC and NMR analyses. The study also leveraged the intrinsic alcoholytic activity of class IV PHA synthases to introduce alkyne end-groups, enabling click-chemistry functionalization. Material characterization showed that the resulting copolymers had altered thermal behavior, lower crystallinity, and improved elasticity compared to standard poly(3-hydroxybutyrate).

Anglický abstrakt

This study investigated wild-type Aneurinibacillus spp. as natural producers of diverse and functionally rich PHA copolymers without any genetic engineering. When grown on glucose with various lactones and diols, the strains incorporated an unusually wide range of monomers, demonstrating high substrate flexibility of their native PHA synthases. Using α-methyl-γ-butyrolactone, the researchers produced a rare α-branched PHA copolymer, confirmed by GC and NMR analyses. The study also leveraged the intrinsic alcoholytic activity of class IV PHA synthases to introduce alkyne end-groups, enabling click-chemistry functionalization. Material characterization showed that the resulting copolymers had altered thermal behavior, lower crystallinity, and improved elasticity compared to standard poly(3-hydroxybutyrate).

Klíčová slova

polyhydroxyalkanoates, click-chemistry, Aneurinibacillus, thermophilic bacteria, heteropolymers

Klíčová slova v angličtině

polyhydroxyalkanoates, click-chemistry, Aneurinibacillus, thermophilic bacteria, heteropolymers

Autoři

ŘEHÁKOVÁ, V.; BUCHTÍKOVÁ, I.; SEDLÁČEK, P.; KALINA, M.; TUŠLOVÁ, K.; OBRUČA, S.

Vydáno

03.10.2025

Nakladatel

NOVA School of Science and Technology

Místo

Lisabon

Strany od

89

Strany do

89

Strany počet

1

URL

https://media.sci-meet.com/www.esbp2025.org/326b420a-8f47-4973-a2a8-2d4010491cbc/BookofAbstractsESBP2025.pdf

BibTex

@misc{BUT199889,
  author="Veronika {Řeháková} and Iva {Buchtíková} and Petr {Sedláček} and Michal {Kalina} and Klára {Tušlová} and Stanislav {Obruča}",
  title="Click Me If You Can: Wild-Type Aneurinibacillus Bacteria as Unlikely Architects of Functional Pha Copolymers",
  year="2025",
  pages="89--89",
  publisher="NOVA School of Science and Technology",
  address="Lisabon",
  url="https://media.sci-meet.com/www.esbp2025.org/326b420a-8f47-4973-a2a8-2d4010491cbc/BookofAbstractsESBP2025.pdf",
  note="Abstract"
}