TISSUE ENGINEERING SCAFFOLDS PREPARED BY PRESSURE INDUCED PHASE SEPARATION FROM POLYHYDROXYALKANOATES.

TISSUE ENGINEERING SCAFFOLDS PREPARED BY PRESSURE INDUCED PHASE SEPARATION FROM POLYHYDROXYALKANOATES.

Abstrakt

Pressure induced phase separation is a promising method of tissue engineering scaffold preparation without the use of organic solvents. Sponge-like scaffolds with high porosity and pore interconnectivity can be prepared. The most common materials are synthetic biodegradable polymers such as polylactide (PLA) or polycaprolactone (PCL)1. Another promising biomaterial for scaffold preparation is poly(3-hydroxybutyrate) from the group of bacterial polyhydroxyalkanoates and its copolymers with other polyhydroxyalkanoate. Its degradation product, β-hydroxybutyric acid is a keto body, naturally found in various tissues. The main disadvantage of polyhydroxyalkanoate compared to synthetic polymers is their low thermal resistance, which might become a problem during PIPS, since it is typically done near the melting temperature. The degradation might be further enhanced in the presence of bioactive glass4. Bioactive glass is used in scaffolds for bone tissue engineering, since it provides sufficient stimulation for bone regeneration without the use of growth factors5. The aim of this work was to test preparation of scaffolds of poly(3-hydroxybutyrate) copolymers for a wide range of applications. Several compositions were prepared, both from synthetic and bacterial polymers. Their mechanical properties and degradation during processing were assessed. Suitable bioactive glass that did not induce too much degradation was found, and a composite prepared. On the other hand, also highly flexible and ductile scaffolds for soft tissue regeneration were prepared.

Pressure induced phase separation is a promising method of tissue engineering scaffold preparation without the use of organic solvents. Sponge-like scaffolds with high porosity and pore interconnectivity can be prepared. The most common materials are synthetic biodegradable polymers such as polylactide (PLA) or polycaprolactone (PCL)1. Another promising biomaterial for scaffold preparation is poly(3-hydroxybutyrate) from the group of bacterial polyhydroxyalkanoates and its copolymers with other polyhydroxyalkanoate. Its degradation product, β-hydroxybutyric acid is a keto body, naturally found in various tissues. The main disadvantage of polyhydroxyalkanoate compared to synthetic polymers is their low thermal resistance, which might become a problem during PIPS, since it is typically done near the melting temperature. The degradation might be further enhanced in the presence of bioactive glass4. Bioactive glass is used in scaffolds for bone tissue engineering, since it provides sufficient stimulation for bone regeneration without the use of growth factors5. The aim of this work was to test preparation of scaffolds of poly(3-hydroxybutyrate) copolymers for a wide range of applications. Several compositions were prepared, both from synthetic and bacterial polymers. Their mechanical properties and degradation during processing were assessed. Suitable bioactive glass that did not induce too much degradation was found, and a composite prepared. On the other hand, also highly flexible and ductile scaffolds for soft tissue regeneration were prepared.

tissue engineering; polyhydroxyalkanoates; bioactive glass; pressure-induced phase separation

tissue engineering; polyhydroxyalkanoates; bioactive glass; pressure-induced phase separation

ŠINDELÁŘ, J.; PŘIKRYL, R.; MASSERA, J.

28.04.2025

Czech Chemical Society

Czech Republic

ISSN 2336-7202

Czech chemical society symposium series

23

2

Česká republika

58

58

72

http://www.ccsss.cz/index.php/ccsss/issue/view/50

50-92-PB