VALORISATION OF NITROGEN-RICH FOOD WASTE MATERIALS FOR BIOMASS PRODUCTION IN BASIDIOMYCETE AND ASCOMYCETE YEASTS

VALORISATION OF NITROGEN-RICH FOOD WASTE MATERIALS FOR BIOMASS PRODUCTION IN BASIDIOMYCETE AND ASCOMYCETE YEASTS

Abstrakt

Yeasts represent a diverse group of microorganisms capable of synthesizing a wide range of nutritionally and biologically valuable compounds, including β-glucans, lipids, ergosterol, ubiquinone, and, in certain species, carotenoid pigments. A key advantage of cultivating microscopic fungi lies in their ability to utilize food industry by-products as nutrient sources, particularly carbon and nitrogen, following appropriate pretreatment. This study aims to evaluate various industrial food waste-derived nitrogen sources—flax hydrolysate, whey protein hydrolysate, spent brewer’s yeast hydrolysate, and feather hydrolysate—by assessing the resulting microbial biomass enriched with valuable metabolites. Additionally, flax oil was introduced into media as carbon source and as a supplementary nutritional stress factor. The ascomycete strain Metchnikowia pulcherrima and the basidiomycete strain Rhodotorula kratochvilovae were cultivated under controlled C/N ratios to ensure comparability of the results. The findings indicate that both yeast strains effectively utilize food waste-derived substrates for biomass production. R. kratochvilovae demonstrated the highest biomass yield when cultivated on medium supplemented with feather hydrolysate as the nitrogen source (10.6 ± 0.1 g/L). M. pulcherrima exhibited maximal biomass production on a medium containing feather hydrolysate and flax oil (10.4 ± 0.2 g/L). Future research will focus on expanding the study to additional yeast strains and scaling up the cultivation process to a laboratory-scale bioreactor to further explore the feasibility of industrial applications.

Yeasts represent a diverse group of microorganisms capable of synthesizing a wide range of nutritionally and biologically valuable compounds, including β-glucans, lipids, ergosterol, ubiquinone, and, in certain species, carotenoid pigments. A key advantage of cultivating microscopic fungi lies in their ability to utilize food industry by-products as nutrient sources, particularly carbon and nitrogen, following appropriate pretreatment. This study aims to evaluate various industrial food waste-derived nitrogen sources—flax hydrolysate, whey protein hydrolysate, spent brewer’s yeast hydrolysate, and feather hydrolysate—by assessing the resulting microbial biomass enriched with valuable metabolites. Additionally, flax oil was introduced into media as carbon source and as a supplementary nutritional stress factor. The ascomycete strain Metchnikowia pulcherrima and the basidiomycete strain Rhodotorula kratochvilovae were cultivated under controlled C/N ratios to ensure comparability of the results. The findings indicate that both yeast strains effectively utilize food waste-derived substrates for biomass production. R. kratochvilovae demonstrated the highest biomass yield when cultivated on medium supplemented with feather hydrolysate as the nitrogen source (10.6 ± 0.1 g/L). M. pulcherrima exhibited maximal biomass production on a medium containing feather hydrolysate and flax oil (10.4 ± 0.2 g/L). Future research will focus on expanding the study to additional yeast strains and scaling up the cultivation process to a laboratory-scale bioreactor to further explore the feasibility of industrial applications.

food waste , circular economy, yeasts

food waste , circular economy, yeasts

HOLUB, J.; LAŠŠOVÁ, E.; OBRAČAJ, J.; ŠPAČKOVÁ, D.; GERSPITZEROVÁ, N.; MÁROVÁ, I.

16.04.2025

AMCA, spol. s r.o.

Praha

978-80-88307-24-2

Book of abstracts of the 12th International Conference on Chemical Technology

153

153

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https://amca-admin.rachel.puxdesign.cz/ICCT/media/content/2025/program/book_of_abstracts_-icct2025.pdf