Detail publikačního výsledku

IMAGING EXTRACELLULAR VESICLES USING ELECTRON MICROSCOPY

KOŽNAROVÁ, S.; VAŠINOVÁ GALIOVÁ, M.; HRSTKA, R.; MRÁZOVÁ, K.; BAČOVSKÝ, J.

Originální název

IMAGING EXTRACELLULAR VESICLES USING ELECTRON MICROSCOPY

Anglický název

IMAGING EXTRACELLULAR VESICLES USING ELECTRON MICROSCOPY

Druh

Konferenční sborník (ne stať)

Originální abstrakt

Extracellular vesicles, particularly exosomes, are nanoscale membrane-bound particles involved in intercellular communication and show significant potential in oncology. Their natural surface proteins provide advantages for targeted drug delivery over synthetic nanomaterials. However, improper selection of source cells or engineering can lead to safety risks. Because exosome morphology may change during cultivation and isolation, reliable imaging is essential. This work focused on finding an efficient imaging method without immunolabelling or cryogenic techniques. After SEM proved unsuitable, STEM and TEM were tested, leading to a successful procedure for rapid visualization and comparison of exosome populations, with no major morphological differences observed.

Anglický abstrakt

Extracellular vesicles, particularly exosomes, are nanoscale membrane-bound particles involved in intercellular communication and show significant potential in oncology. Their natural surface proteins provide advantages for targeted drug delivery over synthetic nanomaterials. However, improper selection of source cells or engineering can lead to safety risks. Because exosome morphology may change during cultivation and isolation, reliable imaging is essential. This work focused on finding an efficient imaging method without immunolabelling or cryogenic techniques. After SEM proved unsuitable, STEM and TEM were tested, leading to a successful procedure for rapid visualization and comparison of exosome populations, with no major morphological differences observed.

Klíčová slova

Extracellular vesicles, exosome, TEM, electron microscopy

Klíčová slova v angličtině

Extracellular vesicles, exosome, TEM, electron microscopy

Autoři

KOŽNAROVÁ, S.; VAŠINOVÁ GALIOVÁ, M.; HRSTKA, R.; MRÁZOVÁ, K.; BAČOVSKÝ, J.

Vydáno

27.11.2025

Nakladatel

Vysoké učení technické v Brně

Místo

Brno

ISBN

978-80-214-6388-2

Strany počet

91

URL

https://www.fch.vut.cz/vav/konference/sok/vystupy/sok-2025-sbornikabstraktu-pdf-p321225

BibTex

@proceedings{BUT201149,
  editor="Simona {Kožnarová} and Michaela {Vašinová Galiová} and  {} and  {} and Jaromír {Bačovský}",
  title="IMAGING EXTRACELLULAR VESICLES USING ELECTRON MICROSCOPY",
  year="2025",
  pages="91",
  publisher="Vysoké učení technické v Brně",
  address="Brno",
  isbn="978-80-214-6388-2",
  url="https://www.fch.vut.cz/vav/konference/sok/vystupy/sok-2025-sbornikabstraktu-pdf-p321225"
}