USING MICROBIAL EXTRACTS TO CREATE COSMETICS PRODUCTS

USING MICROBIAL EXTRACTS TO CREATE COSMETICS PRODUCTS

Abstrakt

This study aimed at the development of cosmetic formulations incorporating microbial extracts while simultaneously exploring the valorisation of food industry by-products, specifically spent coffee grounds, as an alternative cultivation substrate. The primary objective was to convert this waste material into value-added bioactive metabolites with potential cosmetic applications and to characterize these compounds analytically. The investigated metabolites comprised fatty acids, carotenoids, polyphenols, and flavonoids. The antioxidant and antimicrobial properties of the prepared extracts were assessed using the model bacterial strains Escherichia coli and Staphylococcus epidermidis. Based on the obtained results, an appropriate cosmetic formulation containing the microbial extracts was selected. The experimental work included yeast cultivation and co-cultivation with microalgae to enhance the spectrum of bioactive compounds. The microbial strains employed were Phaffia rhodozyma CCY 77-1-1, Cystofilobasidium macerans CCY 10-1-2, Rhodosporidium toruloides CCY 062-002-004, and Chlorella vulgaris CCALA 924. Extracts were prepared from both monocultures and the co-culture of Phaffia rhodozyma and Chlorella vulgaris, with biomass combined in equal proportions (1:1) for the final extract preparation. The resulting cosmetic product was formulated as a body scrub enriched with microbial extracts and residual spent coffee grounds. Coffee oil derived from the waste material was utilized during microbial cultivation, while the remaining solid fraction was incorporated into the scrub formulation, enabling comprehensive waste utilization within the production process.

This study aimed at the development of cosmetic formulations incorporating microbial extracts while simultaneously exploring the valorisation of food industry by-products, specifically spent coffee grounds, as an alternative cultivation substrate. The primary objective was to convert this waste material into value-added bioactive metabolites with potential cosmetic applications and to characterize these compounds analytically. The investigated metabolites comprised fatty acids, carotenoids, polyphenols, and flavonoids. The antioxidant and antimicrobial properties of the prepared extracts were assessed using the model bacterial strains Escherichia coli and Staphylococcus epidermidis. Based on the obtained results, an appropriate cosmetic formulation containing the microbial extracts was selected. The experimental work included yeast cultivation and co-cultivation with microalgae to enhance the spectrum of bioactive compounds. The microbial strains employed were Phaffia rhodozyma CCY 77-1-1, Cystofilobasidium macerans CCY 10-1-2, Rhodosporidium toruloides CCY 062-002-004, and Chlorella vulgaris CCALA 924. Extracts were prepared from both monocultures and the co-culture of Phaffia rhodozyma and Chlorella vulgaris, with biomass combined in equal proportions (1:1) for the final extract preparation. The resulting cosmetic product was formulated as a body scrub enriched with microbial extracts and residual spent coffee grounds. Coffee oil derived from the waste material was utilized during microbial cultivation, while the remaining solid fraction was incorporated into the scrub formulation, enabling comprehensive waste utilization within the production process.

microorganisms, cosmetics, biotechnology, algae, yeast

microorganisms, cosmetics, biotechnology, algae, yeast

P. Sikorová K. Reichertová I. Márová

15.04.2026

ICCT Mikulov

Mikulov

https://amca-admin.rachel.puxdesign.cz/ICCT/media/2026/book_of_abstracts_-icct2026.pdf