Detail publikačního výsledku

Functionalised magnetic nano- and microspheres for molecular diagnostic applications.

RITTICH, B.; ŠPANOVÁ, A.; HORÁK, D.; BENEŠ, M.

Originální název

Functionalised magnetic nano- and microspheres for molecular diagnostic applications.

Anglický název

Functionalised magnetic nano- and microspheres for molecular diagnostic applications.

Druh

Stať ve sborníku v databázi WoS či Scopus

Originální abstrakt

Magnetic microspheres (P(HEMA-co-EDMA), P(HEMA-co-GMA), and PGMA with hydrophilic properties were prepared by single-step dispersion polymerisation. Sterically or electrostatically stabilised colloidal magnetite was obtained by precipitation of Fe2+ and Fe3+ salts and encapsulated by dispersion (co)polymerisation of HEMA and GMA. Several enzymes, such as RNase A, DNase I, proteinase K and Salmonella antibodies were immobilised on the microspheres and their applicability in degradation of bacterial RNA, chromosomal and plasmid DNA, magnetic separation of Salmonella cells or degradation of their intracellular inhibitors was demonstrated. Genomic DNA was successfully isolated from cell lysates on weakly acid derivatives of magnetic P(HEMA-co-EDMA) and P(HEMA-co-GMA) microspheres in the presence of PEG 6000 and sodium chloride.

Anglický abstrakt

Magnetic microspheres (P(HEMA-co-EDMA), P(HEMA-co-GMA), and PGMA with hydrophilic properties were prepared by single-step dispersion polymerisation. Sterically or electrostatically stabilised colloidal magnetite was obtained by precipitation of Fe2+ and Fe3+ salts and encapsulated by dispersion (co)polymerisation of HEMA and GMA. Several enzymes, such as RNase A, DNase I, proteinase K and Salmonella antibodies were immobilised on the microspheres and their applicability in degradation of bacterial RNA, chromosomal and plasmid DNA, magnetic separation of Salmonella cells or degradation of their intracellular inhibitors was demonstrated. Genomic DNA was successfully isolated from cell lysates on weakly acid derivatives of magnetic P(HEMA-co-EDMA) and P(HEMA-co-GMA) microspheres in the presence of PEG 6000 and sodium chloride.

Klíčová slova

nonporous magnetic hydrophilic microspheres; P(HEMA-co-EDMA); P(HEMA-co-GMA); PGMA; enzymes immobilization; PCR-ready DNA; amplification methods

Klíčová slova v angličtině

nonporous magnetic hydrophilic microspheres; P(HEMA-co-EDMA); P(HEMA-co-GMA); PGMA; enzymes immobilization; PCR-ready DNA; amplification methods

Autoři

RITTICH, B.; ŠPANOVÁ, A.; HORÁK, D.; BENEŠ, M.

Vydáno

13.09.2007

Nakladatel

Research Institute of Chemical and Process Enginnering, University of Pannonia

Místo

Veszprém, Hungary

ISBN

978-963-9696-23-5

Kniha

Biopowders; Advances in the Powder Technology

Strany od

116

Strany do

124

Strany počet

9

BibTex

@inproceedings{BUT25379,
  author="Bohuslav {Rittich} and Alena {Španová}",
  title="Functionalised magnetic nano- and microspheres for molecular diagnostic applications.",
  booktitle="Biopowders; Advances in the Powder Technology",
  year="2007",
  number="1",
  pages="116--124",
  publisher="Research Institute of Chemical and Process Enginnering, University of Pannonia",
  address="Veszprém, Hungary",
  isbn="978-963-9696-23-5"
}