Detail publikace

The Application of PCR Methods for Clostridium Identification

ÜRGEOVÁ, B. ŠPANOVÁ, A. RITTICH, B.

Originální název

Typ

článek ve sborníku ve WoS nebo Scopus

Jazyk

angličtina

Originální abstrakt

The presence of butyric acid bacteria and spores in milk causes problems in cheese technology. Some species of genus Clostridium provoke the late-blowing effect which is characterized by high concentration of butyric acid and formation of gas holes in cheese. This work is focused on application of PCR methods for genus and species identification of selected strains of Clostridium. Using genus-specific PCR, the set of 44 DNA samples was identified as DNA of Clostridium genus. Based on species-specific PCRs, it was found out that 7 samples belong to the species Clostridium butyricum and 12 samples belong to the species Clostridium tyrobutyricum. Fifteen samples were positively detected using both species-specific PCRs and therefore they were identified as DNA mixtures from both cultures. Another 10 samples were not classified into tested species. Strains with the hydrogenase (hydA) gene encoding H2-evolving hydrogenase were also searched using PCR. Specific PCR products were detected in 30 strains of the analysed set. All positive strains were identified as species Clostridium butyricum.

Klíčová slova

cheese, PCR, Clostridium butyricum, C. tyrobutyricum, hydA gene

Autoři

ÜRGEOVÁ, B.; ŠPANOVÁ, A.; RITTICH, B.

Rok RIV

2009

Vydáno

7. 10. 2009

Nakladatel

University of SS. Cyril and Methodius in Trnava

Místo

Nám. J. Herdu 2, Trnava

ISBN

978-80-8105-129-6

Kniha

Nova Biotechnologica, Proceedings of the International Conference Applied Natural Sciences 2009

Strany od

258

Strany do

262

Strany počet

BibTex

@inproceedings{BUT29630,
  author="Barbora {Gregušová} and Alena {Španová} and Bohuslav {Rittich}",
  title="The Application of PCR Methods for Clostridium Identification",
  booktitle="Nova Biotechnologica, Proceedings of the International Conference Applied Natural Sciences 2009",
  year="2009",
  pages="258--262",
  publisher="University of SS. Cyril and Methodius in Trnava",
  address="Nám. J. Herdu 2, Trnava",
  isbn="978-80-8105-129-6"
}