Detail publikačního výsledku
Comparison of two different solvents employed for pressurised fluid extraction of stevioside from Stevia rebaudiana : methanol versus water
PÓL, J.; VARAĎOVÁ OSTRÁ, E.; KARÁSEK, P.; ROTH, M.; BENEŠOVÁ, K.; KOTLAŘÍKOVÁ, P.; ČÁSLAVSKÝ, J.
Originální název
Comparison of two different solvents employed for pressurised fluid extraction of stevioside from Stevia rebaudiana : methanol versus water
Anglický název
Comparison of two different solvents employed for pressurised fluid extraction of stevioside from Stevia rebaudiana : methanol versus water
Druh
Článek recenzovaný mimo WoS a Scopus
Originální abstrakt
Pressurised fluid extraction using water or methanol was employed for the extraction of stevioside from Stevia rebaudiana Bertoni. The extraction method was optimised in terms of temperature and duration of the static or the dynamic step. Extracts were analysed by liquid chromatography followed by UV and mass-spectrometric (MS) detections. Thermal degradation of stevioside was the same in both solvents within the range 70 to 160 deg. C. Methanol showed better extraction ability for isolation of stevioside from Stevia rebaudiana leaves than water within the range 110 to 160 deg. C. However, water represents the green alternative to methanol. The limit of detection of stevioside in the extract analysed was 30 ng for UV detection and 2 ng for MS detection.
Anglický abstrakt
Pressurised fluid extraction using water or methanol was employed for the extraction of stevioside from Stevia rebaudiana Bertoni. The extraction method was optimised in terms of temperature and duration of the static or the dynamic step. Extracts were analysed by liquid chromatography followed by UV and mass-spectrometric (MS) detections. Thermal degradation of stevioside was the same in both solvents within the range 70 to 160 deg. C. Methanol showed better extraction ability for isolation of stevioside from Stevia rebaudiana leaves than water within the range 110 to 160 deg. C. However, water represents the green alternative to methanol. The limit of detection of stevioside in the extract analysed was 30 ng for UV detection and 2 ng for MS detection.
Klíčová slova
Pressurised fluid extraction; Liquid chromatography / Mass spectrometry; Stevia rebaudiana; Stevioside
Klíčová slova v angličtině
Pressurised fluid extraction; Liquid chromatography / Mass spectrometry; Stevia rebaudiana; Stevioside
Autoři
PÓL, J.; VARAĎOVÁ OSTRÁ, E.; KARÁSEK, P.; ROTH, M.; BENEŠOVÁ, K.; KOTLAŘÍKOVÁ, P.; ČÁSLAVSKÝ, J.
Vydáno
15.08.2007
Nakladatel
Springer
Místo
Berlin - Heidelberg
ISSN
1618-2642
Periodikum
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Svazek
388
Číslo
8
Stát
Spolková republika Německo
Strany od
1847
Strany do
1857
Strany počet
11
BibTex
@article{BUT43961,
author="Jaroslav {Pól} and Elena {Ostrá} and Pavel {Karásek} and Michal {Roth} and Karolína {Benešová} and Pavla {Kožušníková} and Josef {Čáslavský}",
title="Comparison of two different solvents employed for pressurised fluid extraction of stevioside from Stevia rebaudiana : methanol versus water",
journal="ANALYTICAL AND BIOANALYTICAL CHEMISTRY",
year="2007",
volume="388",
number="8",
pages="1847--1857",
issn="1618-2642"
}