Detail publikačního výsledku

Exploration on the separability of thaumatin by capillary electrophoresis

VESPALCOVÁ, M.; GREGOROVÁ, D.

Originální název

Exploration on the separability of thaumatin by capillary electrophoresis

Anglický název

Exploration on the separability of thaumatin by capillary electrophoresis

Druh

Článek recenzovaný mimo WoS a Scopus

Originální abstrakt

Electrophoretic behaviour of highly basic protein thaumatin was explored in strongly acid (pH 2) and mildly acid (pH 4.5) separation systems using both bare and coated fused silica capillaries. Separation selectivity for thaumatin I, thaumatin II and for other sample constituents was nor sufficient for their baseline separation at pH 2 with uncoated capillary because of separation efficiency, which was markedly lower than common in electrophoretic separations of proteins. Separation selectivity higher up to one order of magnitude has been reached at pH 4.5. Pronounced asymmetry of zones, which deteriorated resolution at this pH, was effectively suppressed by the coating of the capillary wall with a polymer. Electrochromatographic separation systems based on capillaries coated with the layer of either cationic or hydrophilic uncharged polymer appear to be promising for the development of methods for thaumatin analysis.

Anglický abstrakt

Electrophoretic behaviour of highly basic protein thaumatin was explored in strongly acid (pH 2) and mildly acid (pH 4.5) separation systems using both bare and coated fused silica capillaries. Separation selectivity for thaumatin I, thaumatin II and for other sample constituents was nor sufficient for their baseline separation at pH 2 with uncoated capillary because of separation efficiency, which was markedly lower than common in electrophoretic separations of proteins. Separation selectivity higher up to one order of magnitude has been reached at pH 4.5. Pronounced asymmetry of zones, which deteriorated resolution at this pH, was effectively suppressed by the coating of the capillary wall with a polymer. Electrochromatographic separation systems based on capillaries coated with the layer of either cationic or hydrophilic uncharged polymer appear to be promising for the development of methods for thaumatin analysis.

Klíčová slova

Thaumatin; CE; Basic protein; Separation; Electrochromatography

Klíčová slova v angličtině

Thaumatin; CE; Basic protein; Separation; Electrochromatography

Autoři

VESPALCOVÁ, M.; GREGOROVÁ, D.

Rok RIV

2012

Vydáno

01.08.2003

Nakladatel

Wiley-VCH

Místo

Weinheim

ISSN

1615-9306

Periodikum

JOURNAL OF SEPARATION SCIENCE

Svazek

26

Číslo

8

Stát

Spolková republika Německo

Strany od

727

Strany do

734

Strany počet

7

BibTex

@article{BUT46009,
  author="Milena {Vespalcová} and Daniela {Gregorová}",
  title="Exploration on the separability of thaumatin by capillary electrophoresis",
  journal="JOURNAL OF SEPARATION SCIENCE",
  year="2003",
  volume="26",
  number="8",
  pages="727--734",
  issn="1615-9306"
}