Detail publikačního výsledku

Polygalacturonases from potato tubers

OMELKOVÁ, J.

Originální název

Polygalacturonases from potato tubers

Anglický název

Polygalacturonases from potato tubers

Druh

Článek recenzovaný mimo WoS a Scopus

Originální abstrakt

Potato tubers produce small amount of polygalacturonase. Multiple forms of this enzyme were observed with isoelectric points between 4.6 and 7.2 (major form about 6.5). Polygalacturonase isoforms were characterized by their molecular masses, pH optima, optimum of temperature, thermal stability, KM on polymeric substrate and action pattern on polymeric and oligomeric substrate. All forms showed highly random cleavage pattern of pectate and absence of activity towards both di(D-galactosiduronic) acid and tri(D-galactosiduronic) acid, particularly the trimer. Based on these results potato polygalacturonase (endopolygalacturonase, EC 3.2.1.15) can be characterized as an enzyme with unusual degradation of oligomeric substrates yielding trimer as the end product.

Anglický abstrakt

Potato tubers produce small amount of polygalacturonase. Multiple forms of this enzyme were observed with isoelectric points between 4.6 and 7.2 (major form about 6.5). Polygalacturonase isoforms were characterized by their molecular masses, pH optima, optimum of temperature, thermal stability, KM on polymeric substrate and action pattern on polymeric and oligomeric substrate. All forms showed highly random cleavage pattern of pectate and absence of activity towards both di(D-galactosiduronic) acid and tri(D-galactosiduronic) acid, particularly the trimer. Based on these results potato polygalacturonase (endopolygalacturonase, EC 3.2.1.15) can be characterized as an enzyme with unusual degradation of oligomeric substrates yielding trimer as the end product.

Klíčová slova

OLIGOGALACTURONIC ACIDS; ENDOPOLYGALACTURONASE-I; ASPERGILLUS-NIGER; CRYSTAL-STRUCTURE; TOMATO FRUIT; PURIFICATION; PECTINESTERASE; FIRMNESS; ENZYMES; WEEVIL

Klíčová slova v angličtině

OLIGOGALACTURONIC ACIDS; ENDOPOLYGALACTURONASE-I; ASPERGILLUS-NIGER; CRYSTAL-STRUCTURE; TOMATO FRUIT; PURIFICATION; PECTINESTERASE; FIRMNESS; ENZYMES; WEEVIL

Autoři

OMELKOVÁ, J.

Vydáno

12.05.2005

ISSN

0006-3088

Periodikum

BIOLOGIA

Svazek

2005(3)

Číslo

60

Stát

Slovenská republika

Strany od

267

Strany do

273

Strany počet

6

BibTex

@article{BUT46092,
  author="Jiřina {Omelková}",
  title="Polygalacturonases from potato tubers",
  journal="BIOLOGIA",
  year="2005",
  volume="2005(3)",
  number="60",
  pages="267--273",
  issn="0006-3088"
}