Detail publikace

Functionalised magnetic microspheres with hydrophilic properties for molecular diagnostic applications

RITTICH B. ŠPANOVÁ A. HORÁK D.

Originální název

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Magnetic nonporous hydrophilic microspheres poly(2-hydroxyethyl methacrylate-co-ethylene dimethacrylate)-(P(HEMA-co-EDMA), poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate)-P(HEMA-co-GMA), and poly(glycidyl methacrylate)-PGMA were prepared by single-step dispersion polymerisation in the presence of sterically or electrostatically stabilised colloidal magnetite or maghemite. Several enzymes, such as RNase A, DNase I, proteinase K and Salmonella antibodies were immobilised on the microspheres and their applicability in degradation of bacterial RNA, chromosomal and plasmid DNAs, degradation of intracellular PCR inhibitors and immunomagnetic separation of Salmonella cells were demonstrated. Heterogeneous lanthanide complexes of ethylenediaminetetraacetic acid (EDTA) immobilised on insoluble PGMA support and iminodiacetic acid (IDA) immobilised on magnetic P(HEMA-co-EDMA) microspheres were used as catalysts of plasmid DNA cleavage.

Klíčová slova

nonporous magnetic hydrophilic microspheres; P(HEMA-co-EDMA); P(HEMA-co-GMA); PGMA; enzyme immobilization; PCR-ready DNA; amplification methods

Autoři

RITTICH B.; ŠPANOVÁ A.; HORÁK D.

Rok RIV

2009

Vydáno

10. 5. 2009

Nakladatel

Elsevier

Místo

Amsterdam Holandsko

ISSN

0963-9969

Periodikum

FOOD RESEARCH INTERNATIONAL

Ročník

Číslo

Stát

Spojené státy americké

Strany od

493

Strany do

498

Strany počet

BibTex

@article{BUT48518,
  author="RITTICH B. and ŠPANOVÁ A. and HORÁK D.",
  title="Functionalised magnetic microspheres with hydrophilic properties for molecular diagnostic applications",
  journal="FOOD RESEARCH INTERNATIONAL",
  year="2009",
  volume="42",
  number="4",
  pages="493--498",
  issn="0963-9969"
}