Detail publikačního výsledku
Oligogalacturonate hydrolase with unique substrate preference from the pulp of parsley roots
OMELKOVÁ, J.; FLODROVÁ, D.; STRATILOVÁ, E.
Originální název
Oligogalacturonate hydrolase with unique substrate preference from the pulp of parsley roots
Anglický název
Oligogalacturonate hydrolase with unique substrate preference from the pulp of parsley roots
Druh
Článek recenzovaný mimo WoS a Scopus
Originální abstrakt
The main form of pectate hydrolases in the cell wall of parsley roots showed a unique substrate preference of a plant exopolygalacturonase because it clearly preferred the substrates with degree of polymerization about 10. This form was separated from the others, purified and characterized. Enzyme exhibited sharp pH optimum corresponding to pH 4.7, molecular mass 53.5 kDa, and isoelectric point 5.3. It was stable at 50A degrees C in 2-h assay and had optimum of temperature at 60A degrees C (activation energy being 37.0 kJ/mol). The interaction with concanavalin A indicated the glycosylation of enzyme. Substrates were cleaved from the non-reducing end.
Anglický abstrakt
The main form of pectate hydrolases in the cell wall of parsley roots showed a unique substrate preference of a plant exopolygalacturonase because it clearly preferred the substrates with degree of polymerization about 10. This form was separated from the others, purified and characterized. Enzyme exhibited sharp pH optimum corresponding to pH 4.7, molecular mass 53.5 kDa, and isoelectric point 5.3. It was stable at 50A degrees C in 2-h assay and had optimum of temperature at 60A degrees C (activation energy being 37.0 kJ/mol). The interaction with concanavalin A indicated the glycosylation of enzyme. Substrates were cleaved from the non-reducing end.
Klíčová slova
EXO-D-GALACTURONANASE; ASPERGILLUS-NIGER; GLYCOSIDE HYDROLASES; BIOCHEMICAL-CHARACTERIZATION; ENDOPOLYGALACTURONASE-I; CRYSTAL-STRUCTURE; EXOPOLYGALACTURONASE; POLYGALACTURONASE; CARROT; CHROMATOGRAPHY
Klíčová slova v angličtině
EXO-D-GALACTURONANASE; ASPERGILLUS-NIGER; GLYCOSIDE HYDROLASES; BIOCHEMICAL-CHARACTERIZATION; ENDOPOLYGALACTURONASE-I; CRYSTAL-STRUCTURE; EXOPOLYGALACTURONASE; POLYGALACTURONASE; CARROT; CHROMATOGRAPHY
Autoři
OMELKOVÁ, J.; FLODROVÁ, D.; STRATILOVÁ, E.
Rok RIV
2012
Vydáno
02.04.2009
ISSN
0006-3088
Periodikum
BIOLOGIA
Svazek
2009 (64)
Číslo
2
Stát
Slovenská republika
Strany od
228
Strany do
234
Strany počet
7
BibTex
@article{BUT48997,
author="Jiřina {Omelková} and Dana {Flodrová} and Eva {Stratilová}",
title="Oligogalacturonate hydrolase with unique substrate preference from the pulp of parsley roots",
journal="BIOLOGIA",
year="2009",
volume="2009 (64)",
number="2",
pages="228--234",
issn="0006-3088"
}