Detail publikace
Screening of Clostridium strains isolated from cheeses for species identification and formation of fatty acids
RITTICH, B., URGEOVA, B., ŠPANOVA, A., KVASNIČKOVÁ, E., HAVLÍKOVÁ, Š., ČERNÝ V.
Originální název
Screening of Clostridium strains isolated from cheeses for species identification and formation of fatty acids
Typ
abstrakt
Jazyk
angličtina
Originální abstrakt
Non-pathogenic bacterial strains of the Clostridium genus collected in the Dairy Research Institute, Czech Republic, were isolated from cheeses with late blowing disorders. A set of 44 strains was identified into the Clostridium genus using genus-specific PCR. PCRs specific to the species Clostridium butyricum and Clostridium tyrobutyricum were used for species identification. It was found that 7 and 12 strains belonged to the species C. butyricum or C. tyrobutyricum, respectively. Formation of fatty acids (acetic, propionic, and butyric) was studied by capillary isotachophoresis in the cultivation medium. The content of fatty acids was correlated with changes in the cultivation medium's electrical conductance measured with the Rapid Automatic Bacterial Impedance Technique (RABIT). The molecular parameters received were used for correlation with hydrogen production.
Klíčová slova
Clostridium butyricum; Clostridium tyrobutyricum; PCRs; volatile fatty acids
Autoři
RITTICH, B., URGEOVA, B., ŠPANOVA, A., KVASNIČKOVÁ, E., HAVLÍKOVÁ, Š., ČERNÝ V.
Vydáno
14. 9. 2010
Nakladatel
University of Bologna
Místo
Rimini, Itálie
Strany od
PF24
Strany do
PF24
Strany počet
1