Detail publikačního výsledku

Optimization of polymerase chain reaction for detection of live and dead probiotic lactobacillus cells

TRACHTOVÁ, Š.; ŠPANOVÁ, A.; RITTICH, B.

Originální název

Optimization of polymerase chain reaction for detection of live and dead probiotic lactobacillus cells

Anglický název

Optimization of polymerase chain reaction for detection of live and dead probiotic lactobacillus cells

Druh

Abstrakt

Originální abstrakt

The general application of ethidium monoazide (EMA) is based on penetration into dead cells with copromised membrane integrity. DNA bound to EMA cannot be PCR amplified. Experiments were performed using probiotic bacterial strein Lactobacillus paracasei subsp. paracasei CCDM 211/06. The lactobacillus species specific primers were used for DNA amplification. The PCR products (250 bp) were separated using electrophoresis in 1.8 % agarose gel. The method was applied for live and dead lactobacillus cells identification in dairy products.

Anglický abstrakt

The general application of ethidium monoazide (EMA) is based on penetration into dead cells with copromised membrane integrity. DNA bound to EMA cannot be PCR amplified. Experiments were performed using probiotic bacterial strein Lactobacillus paracasei subsp. paracasei CCDM 211/06. The lactobacillus species specific primers were used for DNA amplification. The PCR products (250 bp) were separated using electrophoresis in 1.8 % agarose gel. The method was applied for live and dead lactobacillus cells identification in dairy products.

Klíčová slova

ethidium monoazide; dead and live bacterial cells

Klíčová slova v angličtině

ethidium monoazide; dead and live bacterial cells

Autoři

TRACHTOVÁ, Š.; ŠPANOVÁ, A.; RITTICH, B.

Vydáno

04.06.2008

Nakladatel

Univerzita P.J. Šafárika

Místo

Košice, Slovenská Republika

Kniha

Proceedings o International Probiotic Conference, ISSN 1336-863X

Strany od

55

Strany do

55

Strany počet

1

BibTex

@misc{BUT61084,
  author="Štěpánka {Trachtová} and Alena {Španová} and Bohuslav {Rittich}",
  title="Optimization of polymerase chain reaction for detection of live and dead probiotic lactobacillus cells",
  booktitle="Proceedings o International Probiotic Conference, ISSN 1336-863X",
  year="2008",
  edition="1",
  pages="55--55",
  publisher="Univerzita P.J. Šafárika",
  address="Košice,
Slovenská Republika",
  note="Abstract"
}