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Determination of sulphonamides in wastewater

VÍTEČKOVÁ, H.; MRAVCOVÁ, L.; VÁVROVÁ, M.

Originální název

Determination of sulphonamides in wastewater

Anglický název

Determination of sulphonamides in wastewater

Druh

Audiovizuální tvorba

Originální abstrakt

Sulphonamides are widely used antibiotics in human and veterinary medicine. These compounds have been shown to block biological processes in wastewater treatment plants. Furthermore, bacterial resistence against them has been proven to establish easily. The focus of this research was to developed separation and identification technique for sulphonamides in wastewater. Sulphonamides were concentrated by l/l extraction using ethyl acetate. The HPLC column ZORBAX Eclipse XDB-C18 (150mm x 4.6mm) was used and elution was carried out using gradient mode. As mobile phase was used acetonitrile and 0,1% formic acid in purified water. Sulphonamides were detected using UV absorbance at 270 nm. Average recovery obtained for sulfonamides was 84.1 6.8 %. Limit of quatification (LOQ) for sulfathiazol was 0.5 ng.ml-1 and limit of detection was 0.2 ng.ml-1. The optimized method was used for real samples collected from wastewater treatment plant in University of Veterinary and Pharmaceutical Sciences Brno campus.

Anglický abstrakt

Sulphonamides are widely used antibiotics in human and veterinary medicine. These compounds have been shown to block biological processes in wastewater treatment plants. Furthermore, bacterial resistence against them has been proven to establish easily. The focus of this research was to developed separation and identification technique for sulphonamides in wastewater. Sulphonamides were concentrated by l/l extraction using ethyl acetate. The HPLC column ZORBAX Eclipse XDB-C18 (150mm x 4.6mm) was used and elution was carried out using gradient mode. As mobile phase was used acetonitrile and 0,1% formic acid in purified water. Sulphonamides were detected using UV absorbance at 270 nm. Average recovery obtained for sulfonamides was 84.1 6.8 %. Limit of quatification (LOQ) for sulfathiazol was 0.5 ng.ml-1 and limit of detection was 0.2 ng.ml-1. The optimized method was used for real samples collected from wastewater treatment plant in University of Veterinary and Pharmaceutical Sciences Brno campus.

Klíčová slova

sulphonamides; wastewater; HPLC; DAD

Klíčová slova v angličtině

sulphonamides; wastewater; HPLC; DAD

Autoři

VÍTEČKOVÁ, H.; MRAVCOVÁ, L.; VÁVROVÁ, M.

Vydáno

04.12.2006

Nakladatel

Brno University of Technology, Faculty of Chemistry

Místo

Brno

ISBN

80-214-3320-5

Kniha

the book of abstract

Edice

1

Strany od

224

Strany do

224

Strany počet

1

URL

http://www.fch.vutbr.cz

BibTex

@misc{BUT63484,
  author="Hana {Lisá} and Ludmila {Mravcová} and Milada {Vávrová}",
  title="Determination of sulphonamides in wastewater",
  booktitle="the book of abstract",
  year="2006",
  series="1",
  edition="1",
  pages="224--224",
  publisher="Brno University of Technology, Faculty of Chemistry",
  address="Brno",
  isbn="80-214-3320-5",
  url="http://www.fch.vutbr.cz",
  note="Audiovisual work"
}