Detail publikačního výsledku

Application of real-time PCR for evaluation of magnetic nanoparticles in molecular diagnostics

ŠPANOVÁ, A.; TRACHTOVÁ, Š.; RITTICH, B.

Originální název

Application of real-time PCR for evaluation of magnetic nanoparticles in molecular diagnostics

Anglický název

Application of real-time PCR for evaluation of magnetic nanoparticles in molecular diagnostics

Druh

Abstrakt

Originální abstrakt

In vitro amplification of nucleic acids using the polymerase chain reaction (PCR) has become a powerful diagnostic tool for the analysis of microorganisms. However, the sensitivity of PCR may be reduced when complex biological samples are studied, since various extracellular PCR inhibitors may be present. The problem can be resolved by means of various DNA isolation and purification methods. Magnetic particles can be successfully used for the isolation of DNA from various samples containing PCR inhibitors. Research has been focused on the development of innovative magnetic nanoparticles.

Anglický abstrakt

In vitro amplification of nucleic acids using the polymerase chain reaction (PCR) has become a powerful diagnostic tool for the analysis of microorganisms. However, the sensitivity of PCR may be reduced when complex biological samples are studied, since various extracellular PCR inhibitors may be present. The problem can be resolved by means of various DNA isolation and purification methods. Magnetic particles can be successfully used for the isolation of DNA from various samples containing PCR inhibitors. Research has been focused on the development of innovative magnetic nanoparticles.

Klíčová slova

magnetic particles, polymerase chain reaction, probiotic dairy products

Klíčová slova v angličtině

magnetic particles, polymerase chain reaction, probiotic dairy products

Autoři

ŠPANOVÁ, A.; TRACHTOVÁ, Š.; RITTICH, B.

Rok RIV

2012

Vydáno

22.05.2011

Místo

Pretoria - South Africa

ISBN

978-1-86854-946-7

Kniha

Book of Abstracts, 11th International Conference on Frontiers of Polymers and Advanced Materials, Pretoria

Strany počet

1

BibTex

@misc{BUT72679,
  author="Alena {Španová} and Štěpánka {Trachtová} and Bohuslav {Rittich}",
  title="Application of real-time PCR for evaluation of magnetic nanoparticles in molecular diagnostics",
  booktitle="Book of Abstracts, 11th International Conference on Frontiers of Polymers and Advanced Materials, Pretoria",
  year="2011",
  pages="1",
  address="Pretoria - South Africa",
  isbn="978-1-86854-946-7",
  note="Abstract"
}