Detail publikačního výsledku
Streptavidin-modified magnetic poly(2-hydroxyethyl methacrylate-co-g-lycidyl methacrylate) microspheres for selective isolation of bacterial DNA.
HORÁK, D., ŠPANOVÁ A., TVRDÍKOVÁ J.,RITTICH, B.
Originální název
Streptavidin-modified magnetic poly(2-hydroxyethyl methacrylate-co-g-lycidyl methacrylate) microspheres for selective isolation of bacterial DNA.
Anglický název
Streptavidin-modified magnetic poly(2-hydroxyethyl methacrylate-co-g-lycidyl methacrylate) microspheres for selective isolation of bacterial DNA.
Druh
Článek WoS
Originální abstrakt
The dispersion copolymerization of 2-hydroxyethyl methacrylate (HEMA) with glycidyl methacrylate (GMA) in a toluene/2-methylpropan-1-ol mixture in the presence of Fe3O4 nanoparticles coated with oleic acid, produced monodisperse magnetic poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) (P(HEMA-GMA)) microspheres. Oxirane groups of the microspheres were ammonolyzed and then functionalized with streptavidin using cyanuric chloride. The final product contained 0.67 mg of streptavidin per gram of wet magnetic P(HEMA-GMA) microspheres. The microspheres were characterized by elemental analysis, scanning electron microscopy, IR, UV VIS and atomic absorption spectroscopy The streptavidin-modified magnetic P(HEMA-GMA) microspheres were used for immobilization of biotinylated DNA and subsequent selective isolation of target DNA from complex samples using DNA/DNA hybridisation.
Anglický abstrakt
The dispersion copolymerization of 2-hydroxyethyl methacrylate (HEMA) with glycidyl methacrylate (GMA) in a toluene/2-methylpropan-1-ol mixture in the presence of Fe3O4 nanoparticles coated with oleic acid, produced monodisperse magnetic poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) (P(HEMA-GMA)) microspheres. Oxirane groups of the microspheres were ammonolyzed and then functionalized with streptavidin using cyanuric chloride. The final product contained 0.67 mg of streptavidin per gram of wet magnetic P(HEMA-GMA) microspheres. The microspheres were characterized by elemental analysis, scanning electron microscopy, IR, UV VIS and atomic absorption spectroscopy The streptavidin-modified magnetic P(HEMA-GMA) microspheres were used for immobilization of biotinylated DNA and subsequent selective isolation of target DNA from complex samples using DNA/DNA hybridisation.
Klíčová slova
Glycidyl methacrylate; 2-hydroxyethyl methacrylate; Streptavidin; Biotin; Magnetic Microspheres; DNA
Klíčová slova v angličtině
Glycidyl methacrylate; 2-hydroxyethyl methacrylate; Streptavidin; Biotin; Magnetic Microspheres; DNA
Autoři
HORÁK, D., ŠPANOVÁ A., TVRDÍKOVÁ J.,RITTICH, B.
Rok RIV
2012
Vydáno
30.05.2011
Nakladatel
PERGAMON-ELSEVIER SCIENCE
Místo
Oxford
ISSN
0014-3057
Periodikum
EUROPEAN POLYMER JOURNAL
Svazek
47
Číslo
5
Stát
Spojené království Velké Británie a Severního Irska
Strany od
1090
Strany do
1096
Strany počet
7
BibTex
@article{BUT73688,
author="Bohuslav {Rittich} and Alena {Španová} and Jana {Tvrdíková}",
title="Streptavidin-modified magnetic poly(2-hydroxyethyl methacrylate-co-g-lycidyl methacrylate) microspheres for selective isolation of bacterial DNA.",
journal="EUROPEAN POLYMER JOURNAL",
year="2011",
volume="47",
number="5",
pages="1090--1096",
issn="0014-3057"
}