Alternative procedure for preparation of Lactobacillus cell lysates

Alternative procedure for preparation of Lactobacillus cell lysates

Abstrakt

The Lactobacillus species play an important role in the food industry.They are also very important for human health due to their positive influence of the gastrointestinal tract functions. Identification of the Lactobacillus species is based on the DNA amplification methods. Molecular methods are essential for correct identification of Lactobacillus at the genus or species levels. Polymerase chain reaction (PCR) is common amplification method used in biological laboratories. PCR-ready DNA must be available to amplification. Enzymes and detergents are used for the crude cell lysates preparation. Enzymes disrupt the cell wall and cell proteins and detergents destroy the plasmatic membrane of the cells. DNA is relased into medium and it is available for purification procedure. Lysozyme, sodium dodecyl sulphate (SDS) and proteinase K are the standard components in the bacterial crude lysates preparations. They are relativelly expensive and sophisticated to storage. The aim of this work was find out if enzymes and detergents presented in the washing powders are capable of cell lysis at adequate quality.

The Lactobacillus species play an important role in the food industry.They are also very important for human health due to their positive influence of the gastrointestinal tract functions. Identification of the Lactobacillus species is based on the DNA amplification methods. Molecular methods are essential for correct identification of Lactobacillus at the genus or species levels. Polymerase chain reaction (PCR) is common amplification method used in biological laboratories. PCR-ready DNA must be available to amplification. Enzymes and detergents are used for the crude cell lysates preparation. Enzymes disrupt the cell wall and cell proteins and detergents destroy the plasmatic membrane of the cells. DNA is relased into medium and it is available for purification procedure. Lysozyme, sodium dodecyl sulphate (SDS) and proteinase K are the standard components in the bacterial crude lysates preparations. They are relativelly expensive and sophisticated to storage. The aim of this work was find out if enzymes and detergents presented in the washing powders are capable of cell lysis at adequate quality.

crude lysates, Lactobacillus, proteinase K, SDS, DNA

crude lysates, Lactobacillus, proteinase K, SDS, DNA

FRIČOVÁ, M.; ČUTA, R.; ŠPANOVÁ, A. ; RITTICH, B.

2012

17.08.2011

Česká společnost chemická

Praha

0009-2770

CHEMICKE LISTY

105

18

Česká republika

s1011

s1011

1