PCR-fingerprinting as a method for identification of Saccharomyces yeasts isolated from various Moravian musts

PCR-fingerprinting as a method for identification of Saccharomyces yeasts isolated from various Moravian musts

Abstrakt

In this study, we used PCR fingerprinting method for identification of Saccharomyces genus. In order to species identification, we used and compared 3 microsatelite primers (GTG)5, (GAG)5, (GAC)5 and M13 phage. We identified 40 different collection yeast strains of Saccharomyces genus (from Collection of Yeasts Bratislava CCY) and yeasts isolated from South Moravian musts. We have chosen 2 regions (Velké Pavlovice and Ivaň) and 3 various musts production; conventional, ecological and integrated. Together, we characterized 82 wine yeasts of Saccharomyces genus.

In this study, we used PCR fingerprinting method for identification of Saccharomyces genus. In order to species identification, we used and compared 3 microsatelite primers (GTG)5, (GAG)5, (GAC)5 and M13 phage. We identified 40 different collection yeast strains of Saccharomyces genus (from Collection of Yeasts Bratislava CCY) and yeasts isolated from South Moravian musts. We have chosen 2 regions (Velké Pavlovice and Ivaň) and 3 various musts production; conventional, ecological and integrated. Together, we characterized 82 wine yeasts of Saccharomyces genus.

PCR-fingerprinting, M13, yeasts, wine

PCR-fingerprinting, M13, yeasts, wine

VRÁNOVÁ, D.; ŠURANSKÁ, H.; OMELKOVÁ, J.; AUGUSTOVÁ, K.; VADKERTIOVÁ, R.

2012

29.04.2011

SAS

Bratislava

39th Annual Conference on Yeasts

1336-4839

34th Annual Conference on Yeasts, Book of abstracts

39

1

Slovenská republika

s113

s113

1