Development of laboratory equipment for cultivation of anaerobic microorganisms in inert atmosphere enabling capturing and sampling of gas and cultivation media

článek ve sborníku ve WoS nebo Scopus

angličtina

Cultivation of anaerobic microorganisms require oxygen-free (an inert atmosphere) environment. Some species of microorganisms produce gas during cultivation in addition to metabolites released into the culture medium. For characterization of these microorganisms and their to test their potential for the gas production, it is necessary to capture the gas and then analyze the quantity and composition. The available literature does describe techniques and equipment to capture gases for the purpose of subsequent analysis under conditions of anaerobic atmosphere. Therefore, it was necessary to develop a laboratory equipments, able to capture the gas generated during the cultivations. These bio-reactors must realize these conditions: the possibility of determination of the total amount of generated gas and allow sampling for analysis, be able to fill and maintain an inert atmosphere, to ensure reproductibility of experiments, be simple and cheap. Filling must be done quickly, easily and sterile, with the current provision of an inert atmosphere. In addition to collection of the gas, it must be able to sample medium with culture during and after the experiment. Bioreactor was made from a glass vial with tubing for generated gas and another tubing for injection of culture media and inert atmosphere. Bioreactor is coupled with reservoir of the gas collector. There are sampling points on bioreactor and reservoirt. Developed bioreactors with the required properties have been successfully tested by the real cultivations of bacteria of the genus Clostridium. Gas composition was analyzed by gas chromatography, and the culture medium was monitored in means OD, pH, and selected fatty acids were determined by GC.

laboratory equipments, anaerobic cultivation, gas collection, development of equipment, gas analysis ; analysis of cultivation medium

DVOŘÁK, M.; VESPALCOVÁ, M.; RITTICH, B.; GREGUŠOVÁ, B.; DUBSKÝ, H.

16. 2. 2012

Vysoké učení technické v Brně, Fakulta Chemická

Purkyňova 464/118, 612 00 Brno

978-80-214-4425-6

Studentská odborná konference Chemie a společnost 2011/12 - Sborník příspěvků

1

1

59

64

5