Detail publikačního výsledku
Identification and determination of relatedness of lactobacilli using different DNA amplification methods
TURKOVÁ, K.; RITTICH, B.; ŠPANOVÁ, A.
Originální název
Identification and determination of relatedness of lactobacilli using different DNA amplification methods
Anglický název
Identification and determination of relatedness of lactobacilli using different DNA amplification methods
Druh
Článek recenzovaný mimo WoS a Scopus
Originální abstrakt
Several DNA amplification-based methods were used for identification and evaluation of the relation between lactobacilli isolated from breastfed full-term infant faeces, dairy products and silage. Twenty-seven strains isolated friom infant faeces were identified as Lactobacillus rhamnosus, Lactobacillus gasseri, Lactobacillus paracasei, Lactobacillus fermentum, Lactobacillus salivarius, Lactobacillus plantarum, and Lactobacillus helveticus using ten species-specific PCRs, multiplex PCR, and sequencing of 16SrDNA. Four strains were not identified. A repetitive extragenic palindromic PCR (rep-PCR) and randomly amplified polymorphic DNA PCR (RAPD-PCR) were used for confirmation of species identification. Fingerprints were used for evaluation of the relatedness of lactobacilli. Differences between strains from infant faeces, dairy products, and silage were not detected.
Anglický abstrakt
Several DNA amplification-based methods were used for identification and evaluation of the relation between lactobacilli isolated from breastfed full-term infant faeces, dairy products and silage. Twenty-seven strains isolated friom infant faeces were identified as Lactobacillus rhamnosus, Lactobacillus gasseri, Lactobacillus paracasei, Lactobacillus fermentum, Lactobacillus salivarius, Lactobacillus plantarum, and Lactobacillus helveticus using ten species-specific PCRs, multiplex PCR, and sequencing of 16SrDNA. Four strains were not identified. A repetitive extragenic palindromic PCR (rep-PCR) and randomly amplified polymorphic DNA PCR (RAPD-PCR) were used for confirmation of species identification. Fingerprints were used for evaluation of the relatedness of lactobacilli. Differences between strains from infant faeces, dairy products, and silage were not detected.
Klíčová slova
Lactobacillus, breastfed infants, dairy products, identification, PCR, fingerprinting
Klíčová slova v angličtině
Lactobacillus, breastfed infants, dairy products, identification, PCR, fingerprinting
Autoři
TURKOVÁ, K.; RITTICH, B.; ŠPANOVÁ, A.
Rok RIV
2013
Vydáno
01.09.2012
Nakladatel
Springer
ISSN
0366-6352
Periodikum
Chemical Papers
Svazek
66
Číslo
9
Stát
Slovenská republika
Strany od
842
Strany do
851
Strany počet
10
Plný text v Digitální knihovně
BibTex
@article{BUT93027,
author="Kristýna {Turková} and Bohuslav {Rittich} and Alena {Španová}",
title="Identification and determination of relatedness of lactobacilli using different DNA amplification methods",
journal="Chemical Papers",
year="2012",
volume="66",
number="9",
pages="842--851",
issn="0366-6352"
}