Detail publikačního výsledku

Identification and determination of relatedness of lactobacilli using different DNA amplification methods

TURKOVÁ, K.; RITTICH, B.; ŠPANOVÁ, A.

Originální název

Identification and determination of relatedness of lactobacilli using different DNA amplification methods

Anglický název

Identification and determination of relatedness of lactobacilli using different DNA amplification methods

Druh

Článek recenzovaný mimo WoS a Scopus

Originální abstrakt

Several DNA amplification-based methods were used for identification and evaluation of the relation between lactobacilli isolated from breastfed full-term infant faeces, dairy products and silage. Twenty-seven strains isolated friom infant faeces were identified as Lactobacillus rhamnosus, Lactobacillus gasseri, Lactobacillus paracasei, Lactobacillus fermentum, Lactobacillus salivarius, Lactobacillus plantarum, and Lactobacillus helveticus using ten species-specific PCRs, multiplex PCR, and sequencing of 16SrDNA. Four strains were not identified. A repetitive extragenic palindromic PCR (rep-PCR) and randomly amplified polymorphic DNA PCR (RAPD-PCR) were used for confirmation of species identification. Fingerprints were used for evaluation of the relatedness of lactobacilli. Differences between strains from infant faeces, dairy products, and silage were not detected.

Anglický abstrakt

Several DNA amplification-based methods were used for identification and evaluation of the relation between lactobacilli isolated from breastfed full-term infant faeces, dairy products and silage. Twenty-seven strains isolated friom infant faeces were identified as Lactobacillus rhamnosus, Lactobacillus gasseri, Lactobacillus paracasei, Lactobacillus fermentum, Lactobacillus salivarius, Lactobacillus plantarum, and Lactobacillus helveticus using ten species-specific PCRs, multiplex PCR, and sequencing of 16SrDNA. Four strains were not identified. A repetitive extragenic palindromic PCR (rep-PCR) and randomly amplified polymorphic DNA PCR (RAPD-PCR) were used for confirmation of species identification. Fingerprints were used for evaluation of the relatedness of lactobacilli. Differences between strains from infant faeces, dairy products, and silage were not detected.

Klíčová slova

Lactobacillus, breastfed infants, dairy products, identification, PCR, fingerprinting

Klíčová slova v angličtině

Lactobacillus, breastfed infants, dairy products, identification, PCR, fingerprinting

Autoři

TURKOVÁ, K.; RITTICH, B.; ŠPANOVÁ, A.

Rok RIV

2013

Vydáno

01.09.2012

Nakladatel

Springer

ISSN

0366-6352

Periodikum

Chemical Papers

Svazek

66

Číslo

9

Stát

Slovenská republika

Strany od

842

Strany do

851

Strany počet

10

BibTex

@article{BUT93027,
  author="Kristýna {Turková} and Bohuslav {Rittich} and Alena {Španová}",
  title="Identification and determination of relatedness of lactobacilli using different DNA amplification methods",
  journal="Chemical Papers",
  year="2012",
  volume="66",
  number="9",
  pages="842--851",
  issn="0366-6352"
}