THE USE OF NATURAL EXTRACTS IN COSMETICS AND SUBSEQUENT DETECTION OF PLANT DNA USING MOLECULAR DIAGNOSTIC METHODS

abstrakt

angličtina

It is known that oxidation reactions could produce free radicals, which can start chain reactions that will damage skin cells. Increasing the amount of free radicals could initiate the wrinkling, photoaging, elastosis, drying, and pigmentation of the skin [1]. Plants can be used as sources of broad spectrum of active compounds. Some of them have antioxidant activity and are preferably used in cosmetics for improving skin care [2]. Presented work is focused on application of natural extracts in cosmetics. Firstly, water and ethanol extracts were prepared. Spectrophotometric analysis of active compounds has been done in terms of polyphenols, flavonoids in green beans extract from Coffea arabica and carotenoids and chlorophylls in microalgae Arthrospira and Dunaliella salina extracts. Antioxidant activity was evaluated in extracts from both natural sources. For better stability in cosmetic formulation, the extracts were encapsulated into liposome particles. Encapsulation efficiency was determined by total polyphenols or total carotenoids entrapped in particles. Particle size and size distribution were analyzed by dynamic light scattering and colloid stability of particles by zeta-potential. Some emulsions of the oil in water type with natural extracts were prepared. Analytical centrifuge was used for testing stability of resulting emulsion. Finally, the prepared emulsions with plant extracts were evaluated by molecular diagnostic methods. Our goal was to prepare emulsions enriched by active compounds from green coffee beans and micro algae to diminish free radicals effect on the skin. Encapsulation of extracts into liposomes was successful and particles were stable and suitable for further application. Analytical centrifuge showed that prepared emulsions were stable and potentially applicable as skin care products. Analysis of plant DNA isolated from green coffee beans, microalgae and prepared extracts and emulsions showed that isolated DNA was in quality allowing its PCR amplification. Using the PCR analysis, the presence of added ingredient of natural plant origin was verified. References [1] I. Kusumawati, G. Indrayanto, Studies in Natural Products Chemistry 40(2013) 485-505. [2] M. B. Ariede, T. M. Candido, Algal Research 25 (2017) 483-487. Acknowledgement This work was also supported by the project “Materials Research Centre at FCH BUT—Sustainability and Development” no. LO1211 of the Ministry of Education, Youth and Sports of the Czech Republic.

natural extracts, encapsulation, cosmetic products, PCR

PAVELKOVÁ, R.; ROMANOVSKÁ, D.; PLACHÁ, M.; PLÁŠKOVÁ, A.; MATOUŠKOVÁ, P.; HÁRONIKOVÁ, A.; MÁROVÁ, I.

12. 9. 2018

Vysoké učení technické v Brně, Fakulta chemická, Purkyňova 464/118, 612 00 Brno

Brno

978-80-214-5488-0

7th Meeting on Chemistry and Life 2018. Book of abstracts

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141

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