Detail publikačního výsledku

The first insight on changes in PHA granules after extraction from extremophilic microorganisms

MRÁZOVÁ, K.; NOVÁČKOVÁ, I.; KOUŘILOVÁ, X.; OBRUČA, S.; KRZYŽÁNEK, V.

Originální název

The first insight on changes in PHA granules after extraction from extremophilic microorganisms

Anglický název

The first insight on changes in PHA granules after extraction from extremophilic microorganisms

Druh

Abstrakt

Originální abstrakt

Polyhydroxyalkanoates (PHA) are microbial polyesters that are accumulated in form of intracellular granules by many prokaryotic organisms. One of the interesting properties of PHA granules is their extreme elasticity. It was found out that PHA stay elastic even at temperatures of liquid nitrogen. In the experiment, cells frozen in liquid nitrogen were freeze-fractured and imaged in a scanning electron microscope with cryostage (cryoSEM). PHA could be then observed sticking out of the fractured cells. In our work, we focused on the extraction of PHA from halophilic Halomonas halophila and thermophilic Schlegelella thermodepolymerans and subsequently on the study of changes in their morphology and elastic properties after extraction using cryoSEM.

Anglický abstrakt

Polyhydroxyalkanoates (PHA) are microbial polyesters that are accumulated in form of intracellular granules by many prokaryotic organisms. One of the interesting properties of PHA granules is their extreme elasticity. It was found out that PHA stay elastic even at temperatures of liquid nitrogen. In the experiment, cells frozen in liquid nitrogen were freeze-fractured and imaged in a scanning electron microscope with cryostage (cryoSEM). PHA could be then observed sticking out of the fractured cells. In our work, we focused on the extraction of PHA from halophilic Halomonas halophila and thermophilic Schlegelella thermodepolymerans and subsequently on the study of changes in their morphology and elastic properties after extraction using cryoSEM.

Klíčová slova

PHA, freeze-fracture, cryo SEM, Halomonas halophila, Schlegelella thermodepolymerans

Klíčová slova v angličtině

PHA, freeze-fracture, cryo SEM, Halomonas halophila, Schlegelella thermodepolymerans

Autoři

MRÁZOVÁ, K.; NOVÁČKOVÁ, I.; KOUŘILOVÁ, X.; OBRUČA, S.; KRZYŽÁNEK, V.

Rok RIV

2022

Vydáno

13.09.2021

Kniha

Microscopy 2021: Conference

Edice

1

Strany od

81

Strany do

81

Strany počet

1

BibTex

@misc{BUT175387,
  author="Kateřina {Mrázová} and Ivana {Nováčková} and Xenie {Kouřilová} and Stanislav {Obruča} and Vladislav {Krzyžánek}",
  title="The first insight on changes in PHA granules after extraction from extremophilic microorganisms",
  booktitle="Microscopy 2021: Conference",
  year="2021",
  series="1",
  edition="1",
  pages="81--81",
  note="Abstract"
}