Detail publikace

Autophagy modulators influence the content of important signalling molecules in PS-positive extracellular vesicles

HÁNĚLOVÁ, K. RAUDENSKÁ, M. KRATOCHVÍLOVÁ, M. NAVRÁTIL, J. VIČAR, T. BUGAJOVÁ, M. GUMULEC, J. MASAŘÍK, M. BALVAN, J.

Originální název

Autophagy modulators influence the content of important signalling molecules in PS-positive extracellular vesicles

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Extracellular vesicles (EVs) are important mediators of intercellular communication in the tumour microenvironment. Many studies suggest that cancer cells release higher amounts of EVs exposing phosphatidylserine (PS) at the surface. There are lots of interconnections between EVs biogenesis and autophagy machinery. Modulation of autophagy can probably affect not only the quantity of EVs but also their content, which can deeply influence the resulting pro-tumourigenic or anticancer effect of autophagy modulators. In this study, we found that autophagy modulators autophinib, CPD18, EACC, bafilomycin A1 (BAFA1), 3-hydroxychloroquine (HCQ), rapamycin, NVP-BEZ235, Torin1, and starvation significantly alter the composition of the protein content of phosphatidylserine-positive EVs (PS-EVs) produced by cancer cells. The greatest impact had HCQ, BAFA1, CPD18, and starvation. The most abundant proteins in PS-EVs were proteins typical for extracellular exosomes, cytosol, cytoplasm, and cell surface involved in cell adhesion and angiogenesis. PS-EVs protein content involved mitochondrial proteins and signalling molecules such as SQSTM1 and TGF ss 1 pro-protein. Interestingly, PS-EVs contained no commonly determined cytokines, such as IL-6, IL-8, GRO-a, MCP-1, RANTES, and GM-CSF, which indicates that secretion of these cytokines is not predominantly mediated through PS-EVs. Nevertheless, the altered protein content of PS-EVs can still participate in the modulation of the fibroblast metabolism and phenotype as p21 was accumulated in fibroblasts influenced by EVs derived from CPD18-treated FaDu cells. The altered protein content of PS-EVs (data are available via ProteomeXchange with identifier PXD037164) also provides information about the cellular compartments and processes that are affected by the applied autophagy modulators.

Klíčová slova

Extracellular vesicles; Phosphatidylserine-positive extracellular vesicles; Autophagy; Autophagy modulation; Head and neck cancer; Proteomics; SQSTM1; Senescence; p21; Autophinib; CPD18; EACC; Bafilomycin A1; 3-hydroxychloroquine; Rapamycin; NVP-BEZ235; Torin1; Starvation

Autoři

HÁNĚLOVÁ, K.; RAUDENSKÁ, M.; KRATOCHVÍLOVÁ, M.; NAVRÁTIL, J.; VIČAR, T.; BUGAJOVÁ, M.; GUMULEC, J.; MASAŘÍK, M.; BALVAN, J.

Vydáno

24. 5. 2023

Nakladatel

BMC

Místo

LONDON

ISSN

1478-811X

Periodikum

Cell Communication and Signaling

Ročník

21

Číslo

1

Stát

Spojené království Velké Británie a Severního Irska

Strany od

1

Strany do

21

Strany počet

21

URL

https://biosignaling.biomedcentral.com/articles/10.1186/s12964-023-01126-z

Plný text v Digitální knihovně

http://hdl.handle.net/11012/213627

BibTex

@article{BUT183987,
  author="Klára {Hánělová} and Martina {Raudenská} and Monika {Kratochvílová} and Jiří {Navrátil} and Tomáš {Vičar} and Mária {Bugajová} and Jaromír {Gumulec} and Michal {Masařík} and Jan {Balvan}",
  title="Autophagy modulators influence the content of important signalling molecules in PS-positive extracellular vesicles",
  journal="Cell Communication and Signaling",
  year="2023",
  volume="21",
  number="1",
  pages="1--21",
  doi="10.1186/s12964-023-01126-z",
  issn="1478-811X",
  url="https://biosignaling.biomedcentral.com/articles/10.1186/s12964-023-01126-z"
}