Detail publikace

Engineering Conductive Hydrogels with Tissue-like Properties: A 3D Bioprinting and Enzymatic Polymerization Approach

Li, CB. Naeimipour, S. Boroojeni, FR. Abrahamsson, T. Strakosas, X. Yi, YPQ. Rilemark, R. Lindholm, C. Perla, VK. Musumeci, C. Li, YY. Biesmans, H. Savvakis, M. Olsson, E. Tybrandt, K. Donahue, MJ. Gerasimov, JY. Selegård, R. Berggren, M. Aili, D. Simon, DT

Originální název

Engineering Conductive Hydrogels with Tissue-like Properties: A 3D Bioprinting and Enzymatic Polymerization Approach

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Hydrogels are promising materials for medical devices interfacing with neural tissues due to their similar mechanical properties. Traditional hydrogel-based bio-interfaces lack sufficient electrical conductivity, relying on low ionic conductivity, which limits signal transduction distance. Conducting polymer hydrogels offer enhanced ionic and electronic conductivities and biocompatibility but often face challenges in processability and require aggressive polymerization methods. Herein, we demonstrate in situ enzymatic polymerization of pi-conjugated monomers in a hyaluronan (HA)-based hydrogel bioink to create cell-compatible, electrically conductive hydrogel structures. These structures were fabricated using 3D bioprinting of HA-based bioinks loaded with conjugated monomers, followed by enzymatic polymerization via horseradish peroxidase. This process increased the hydrogels' stiffness from about 0.6 to 1.5 kPa and modified their electroactivity. The components and polymerization process were well-tolerated by human primary dermal fibroblasts and PC12 cells. This work presents a novel method to fabricate cytocompatible and conductive hydrogels suitable for bioprinting. These hybrid materials combine tissue-like mechanical properties with mixed ionic and electronic conductivity, providing new ways to use electricity to influence cell behavior in a native-like microenvironment. This study introduces a novel method to enhance hydrogel conductivity and biocompatibility for biomedical applications. By using in situ enzymatic polymerization of pi-conjugated monomers within a hyaluronan-based hydrogel bioink, followed by 3D bioprinting, the resulting hydrogels exhibit improved stiffness, electroactivity, and cytocompatibility. These conductive hydrogels provide a versatile platform for advanced 3D cell culture and neural engineering.image (c) 2024 WILEY-VCH GmbH

Klíčová slova

3D printing; cell scaffold; conducting polymer; in vitro; polymerization

Autoři

Li, CB.; Naeimipour, S.; Boroojeni, FR.; Abrahamsson, T.; Strakosas, X.; Yi, YPQ.; Rilemark, R.; Lindholm, C.; Perla, VK.; Musumeci, C.; Li, YY.; Biesmans, H.; Savvakis, M.; Olsson, E.; Tybrandt, K.; Donahue, MJ.; Gerasimov, JY.; Selegård, R.; Berggren, M.; Aili, D.; Simon, DT

Vydáno

1. 11. 2024

Nakladatel

WILEY

Místo

HOBOKEN

ISSN

2688-4046

Periodikum

SMALL SCIENCE

Ročník

4

Číslo

11

Stát

Spojené státy americké

Strany od

1

Strany do

12

Strany počet

12

URL

https://onlinelibrary.wiley.com/doi/10.1002/smsc.202400290

Plný text v Digitální knihovně

http://hdl.handle.net/11012/250835

BibTex

@article{BUT189712,
  author="Changbai {Li} and Sajjad {Naeimipour} and Fatemeh {Rasti Boroojeni} and Tobias {Abrahamsson} and Xenofon {Strakosas} and Yangpeiqi {Yi} and Rebecka {Rilemark} and Caroline {Lindholm} and Venkata K. {Perla} and Chiara {Musumeci} and Yuyang {Li} and Hanne {Biesmans} and Marios {Savvakis} and Eva {Olsson} and Klas {Tybrandt} and Mary {Donahue} and Jennifer Y. {Gerasimov} and Robert {Selegård} and Magnus {Berggren} and Daniel {Aili} and Daniel T. {Simon}",
  title="Engineering Conductive Hydrogels with Tissue-like Properties: A 3D Bioprinting and Enzymatic Polymerization Approach",
  journal="SMALL SCIENCE",
  year="2024",
  volume="4",
  number="11",
  pages="12",
  doi="10.1002/smsc.202400290",
  issn="2688-4046",
  url="https://onlinelibrary.wiley.com/doi/10.1002/smsc.202400290"
}