Detail publikace

In vitro evaluation of 3D-printed conductive chitosan–polyaniline scaffolds with exosome release for enhanced angiogenesis and cardiomyocyte protection

HASHEMI, A. EZATI, M. ZUMBERG, I. CHMELÍKOVÁ, L. FOHLEROVÁ, Z. PROVAZNÍK, V.

Originální název

In vitro evaluation of 3D-printed conductive chitosan–polyaniline scaffolds with exosome release for enhanced angiogenesis and cardiomyocyte protection

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Myocardial infarction (MI) often results in significant damage to heart tissues, leading to cardiac dysfunction, fibrosis, and diminished cell–cell communication. Exosomes (EXOs) from stem cells show great potential in promoting tissue repair and angiogenesis, but their rapid clearance and degradation in vivo limit therapeutic efficacy. Here, we introduce a 3D-printed in vitro scaffold using a conductive biomaterial ink composed of chitosan (CS) and polyaniline (PANI). This scaffold combines the bioactivity of EXOs with the conductive properties of PANI to protect cardiac cells under ischemic stress. Using an in vitro hypoxia/reoxygenation (H/R) model with HL-1 cardiomyocytes, we simulated key aspects of myocardial ischemia-reperfusion injury. The addition of PANI improved the electrical conductivity of the scaffold, which was essential for enhancing cardiomyocyte viability and intercellular connectivity under hypoxic conditions. EXOs significantly promoted angiogenic activity in vitro, as evidenced by enhanced human umbilical vein endothelial cell (HUVEC) migration and robust tube formation, highlighting their role in stimulating new blood vessel growth. Molecular analyses revealed that EXOs positively influence processes such as angiogenesis and inflammation regulation in HL-1 cells. Additionally, EXOs improved HUVEC migration, emphasizing their pro-angiogenic role. These findings indicate that combining PANI and EXOs in a 3D-printed scaffold yields synergistic benefits, improving cardiomyocyte function and promoting endothelial angiogenesis in vitro, thereby providing insights for future cardiac repair strategies.

Klíčová slova

Electroconductive biomaterial ink, 3D bioprinted scaffolds, chitosan, polyaniline, exosomes, HL-1 cardiomyocytes, hypoxia/reoxygenation model, myocardial infarction, cardiac tissue regeneration.

Autoři

HASHEMI, A.; EZATI, M.; ZUMBERG, I.; CHMELÍKOVÁ, L.; FOHLEROVÁ, Z.; PROVAZNÍK, V.

Vydáno

20. 5. 2025

Nakladatel

Royal Society of Chemistry

Místo

Cambridge, England

ISSN

2046-2069

Periodikum

RSC Advances

Ročník

15

Číslo

21

Stát

Spojené království Velké Británie a Severního Irska

Strany od

16826

Strany do

16844

Strany počet

19

URL

https://pubs.rsc.org/en/content/articlelanding/2025/ra/d5ra02940f

Plný text v Digitální knihovně

http://hdl.handle.net/11012/251330

BibTex

@article{BUT197944,
  author="Amir {Hashemi} and Masoumeh {Ezati} and Inna {Zumberg} and Larisa {Chmelíková} and Zdenka {Fohlerová} and Valentýna {Provazník}",
  title="In vitro evaluation of 3D-printed conductive chitosan–polyaniline scaffolds with exosome release for enhanced angiogenesis and cardiomyocyte protection",
  journal="RSC Advances",
  year="2025",
  volume="15",
  number="21",
  pages="16826--16844",
  doi="10.1039/D5RA02940F",
  issn="2046-2069",
  url="https://pubs.rsc.org/en/content/articlelanding/2025/ra/d5ra02940f"
}