The effect of liposomal pro-healing protein on the rheological properties of mucoadhesive injectable hydrogel

abstrakt

angličtina

More significant wounds in the mouth formed after tooth extraction or plastic surgeries are often accompanied by various microorganisms, preventing the wound closure, and prolonging the healing process. To heal these wounds, injectable mucoadhesive material is ideal for promoting wound closure and regeneration. This work aims to prepare an injectable mucoadhesive thermosensitive drug delivery system enriched with pro-healing protein – stable fibroblast growth factor-2 (FGF2-STAB®) and antibacterial enzyme Lysozyme. These active ingredients are further encapsulated into liposomal nanoparticles to prevent protein-matrix interaction and hydrolytic degradation. The mucoadhesive injectable hydrogel is based on poly(lactide-co-glycolide) and poly(ethylene glycol) copolymer (PLGA-PEG-PLGA) functionalized with itaconic acid (ITA), which embodies the mucoadhesive functional groups to the polymer together with the blue-light cross-linkable double bonds. Nanoliposomes with effectively encapsulated pro-healing protein have been successfully incorporated into the PLGA-PEG-PLGA/ITA hydrogel by simple mixing. The liposomes exhibit diameters around 100 nm and low polydispersity. The focus was on investigating the rheological behaviour, especially the gel-sol transition, of the samples containing protein and encapsulated liposomal nanoparticles together and separately. The temperature ramp tests from 25 to 55 °C were performed using parallel-plate geometry at a heating rate of 0.5 °C/min for more sensitive measurement. The results were compared to those of an unfilled matrix to judge the effect caused by the present nanoparticles. It was determined that the nanoparticles tend to decrease the sol-gel transition by 1 °C, whereas the FGF2-STAB® itself tends to shift the gelation point to higher temperatures.

encapsulation, liposome, rheology, PLGA-PEG-PLGA

SEVRIUGINA, V.; KADLECOVÁ, Z.; VOJTOVÁ, L.

28. 8. 2022

Materials Research Society of Serbia

978-86-919111-7-1

Program and Book of Abstracts

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https://www.mrs-serbia.org.rs/images/2022/YUCOMAT_2022-XII_WRTCS.pdf