3D Printed calcium phosphate cement scaffolds loaded with liposomal antibacterial enzymes for the prevention of osteomyelitis

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To treat large-scale bone defects, three-dimensional (3D) printing has been in focus in recent years due to the possibility of fabricating patient-specific bioactive and porous scaffolds1 . When the 3D printed implant begins to resorb, the osteointegration process is often accompanied by several microorganisms that cause a bone infection known as osteomyelitis2 . By combining the ceramic phosphate powder, thermosensitive copolymer, and enzymes, we obtain the bio-ink suitable for 3D printing of bone implants with controllable degradation rate, mechanical properties, pore size, and antibacterial activity. Therefore, as the calcium phosphate cement (CPC) implant resorbs, it suppresses the growth of undesirable microorganisms. Our previous study discovered that antibacterial enzymes incorporated into the bio-ink do not release from the implants in more significant amounts than expected. This probably results from the CPC structure as the enzymes are “locked” within the CPC structure. In this project, the antibacterial enzyme Lysozyme is encapsulated into liposomes with diameters around 100 nm, providing additional chemical and physical protection to the enzyme during the implant preparation and curing. Liposomes prepared by an optimized “heating method” exhibit a low polydispersity index and diameters around 97.8±4.8 nm and zeta potential of -15.2±3.2 mV (n=3). The encapsulation efficiency of Lysozyme in these formulas is around 64 %. It was determined that Lysozyme is released from such liposomes in a first-order release mechanism. The release of the liposome-Lysozyme system from the CPC scaffolds is currently being evaluated.

Calcium phosphate cement, liposomes, osteomyelitis, Lysozyme

KADLECOVÁ, Z.; HLINÁKOVÁ, K.; VOJTOVÁ, L.

29. 8. 2022

Materials Research Society of Serbia

Belgrade

978-86-919111-7-1

Program and Book of Abstracts TWENTY-THIRD ANNUAL CONFERENCE YUCOMAT 2022 & TWELFTH WORLD ROUND TABLE CONFERENCE ON SINTERING XII WRTCS

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https://www.mrs-serbia.org.rs/images/2022/YUCOMAT_2022-XII_WRTCS.pdf