Detail publikace

Biomimetic polydopamine-modified nanofibrous bilayer scaffolds used for full-thickness skin regeneration in a porcine animal model

VOJTOVÁ, L. VERČIMÁKOVÁ, K. PAVLIŇÁKOVÁ, V. POLÁČEK, P. MICHLOVSKÁ, L. BLAHNOVÁ, V. FILOVA, E. KNOZ, M. LIPOVÝ, B. HOLOUBEK, J. FALDYNA, M. PAVLOVSKÝ, Z. VÍCENOVÁ, M. CVANOVÁ, M. JARKOVSKÝ, J.

Originální název

Biomimetic polydopamine-modified nanofibrous bilayer scaffolds used for full-thickness skin regeneration in a porcine animal model

Typ

přednáška

Jazyk

angličtina

Originální abstrakt

Treatment of complete loss of skin thickness requires expensive cell-laden materials and limited skin grafts. In this work, we present a cell-free biomimetic bilayer scaffold based on the cross-linked protein-polysaccharide biopolymeric network modified with an interpenetrated polydopamine (PDA) designed to imitate a bottom layer of the missing dermis and an upper layer of a thin basement membrane (BM). The microbial-protective alternate dermis layer is made from freeze-dried bovine type I collagen and antimicrobial chitosan (Coll/Chit) or collagen and an antimicrobial calcium salt of oxidized cellulose (Coll/CaOC). An alternate antimicrobial basement membrane layer is made from amphiphilic nanofibrous electrospun gelatin (Gel), polycaprolactone (PCL), and CaOC. We have found that PDA significantly improved the elasticity and strength of collagen-polysaccharide-based scaffolds, which favorably affected their swelling capacity, porosity, and stability in the physiological environment. Moreover, based on the in vitro evaluation, PDA significantly supported and maintained metabolic activity, proliferation, and viability of the murine fibroblast cell lines. The in vivo experiment carried out in a domestic Large white pig model resulted in the expression of pro-inflammatory cytokines in the first 1–2 weeks, however, in the middle and later stages, PDA caused a reduction in inflammation with the expression of the anti-inflammatory molecule IL10 and the expansion of the transforming growth factor β, which could support the formation of fibroblasts. Similarities in treatment with native porcine skin suggested that the fully resorbable bioactive biomimetic bilayer can be used as an implant for full-thickness skin wounds and thus eliminate the use of skin grafts.

Klíčová slova

Wound dressing, scaffold, collagen, chitosan, dermis, regenerative medicine, drug release, bioactive molecule

Autoři

VOJTOVÁ, L.; VERČIMÁKOVÁ, K.; PAVLIŇÁKOVÁ, V.; POLÁČEK, P.; MICHLOVSKÁ, L.; BLAHNOVÁ, V.; FILOVA, E.; KNOZ, M.; LIPOVÝ, B.; HOLOUBEK, J.; FALDYNA, M.; PAVLOVSKÝ, Z.; VÍCENOVÁ, M.; CVANOVÁ, M.; JARKOVSKÝ, J.

Vydáno

27. 3. 2023

Místo

International Confrence on Materials Science and Engineering, March 27-28, 2023, Paris, France

Strany počet

1

BibTex

@misc{BUT187007,
  author="Lucy {Vojtová} and Katarína {Verčimáková} and Veronika {Pavliňáková} and Petr {Poláček} and Lenka {Michlovská} and Veronika Hefka {Blahnová} and Eva {Filova} and Martin {Knoz} and Břetislav {Lipový} and Jakub {Holoubek} and Martin {Faldyna} and Zdeněk {Pavlovský} and Monika {Vícenová} and Michaela {Cvanová} and Jiří {Jarkovský}",
  title="Biomimetic polydopamine-modified nanofibrous bilayer scaffolds used for full-thickness skin regeneration in a porcine animal model",
  year="2023",
  pages="1",
  address="International Confrence on Materials Science and Engineering, March 27-28, 2023, Paris, France",
  note="lecture"
}