Detail publikace

Chromatin Immunoprecipitation Reveals p53 Binding to G-Quadruplex DNA Sequences in Myeloid Leukemia Cell Lines

KRATOCHVILOVÁ, L. DINOVÁ, A. VALKOVÁ, N. DOBROVOLNÁ, M. SÁNCHES-MURCIA, PA. BRÁZDA, V.

Originální název

Chromatin Immunoprecipitation Reveals p53 Binding to G-Quadruplex DNA Sequences in Myeloid Leukemia Cell Lines

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Clarifying functions of the p53 protein is a crucial aspect of cancer research. We analyzed the binding sites of p53 wild-type (WT) protein and its oncologically significant mutants and evaluated their transactivation properties using a functional yeast assay. Unlike the binding sites as determined in myeloid leukemia cell lines by chromatin immunoprecipitation of p53-R175H, p53-Y220C, p53-M237I, p53-R248Q, and p53-R273H mutants, the target sites of p53-WT and p53-R282W were significantly associated with putative G-quadruplex sequences (PQSs). Guanine-quadruplex (G-quadruplex or G4) formation in these sequences was evaluated by using a set of biophysical methods. G4s can modulate gene expression induced by p53. At low p53 expression level, PQS upstream of the p53-response element (RE) leads to greater gene expression induced by p53-R282W compared to that for the RE without PQS. Meanwhile, p53-WT protein expression is decreased by the PQS presence. At a high p53 expression level, the presence of PQS leads to a decreased expression of the reporter regardless of the distance and localization of the G4 from the RE.

Klíčová slova

G-quadruplexes; p53 proteins; p53 binding sites; transcription modulation; DNA structures

Autoři

KRATOCHVILOVÁ, L.; DINOVÁ, A.; VALKOVÁ, N.; DOBROVOLNÁ, M.; SÁNCHES-MURCIA, PA.; BRÁZDA, V.

Vydáno

12. 2. 2025

Nakladatel

AMER CHEMICAL SOC

Místo

WASHINGTON

ISSN

2694-2437

Periodikum

ACS Bio & Med Chem Au

Ročník

5

Číslo

2

Stát

Spojené státy americké

Strany od

283

Strany do

298

Strany počet

16

URL

https://pubmed.ncbi.nlm.nih.gov/40255281/

BibTex

@article{BUT197998,
  author="KRATOCHVILOVÁ, L. and DINOVÁ, A. and VALKOVÁ, N. and DOBROVOLNÁ, M. and SÁNCHES-MURCIA, PA. and BRÁZDA, V.",
  title="Chromatin Immunoprecipitation Reveals p53 Binding to G-Quadruplex DNA Sequences in Myeloid Leukemia Cell Lines",
  journal="ACS Bio & Med Chem Au",
  year="2025",
  volume="5",
  number="2",
  pages="283--298",
  doi="10.1021/acsbiomedchemau.4c00124",
  issn="2694-2437",
  url="https://pubmed.ncbi.nlm.nih.gov/40255281/"
}