Detail publikačního výsledku

Finishing the S.pombe genome deletion project. An update.

ČARNECKÁ, M.; MÁROVÁ, I.

Originální název

Finishing the S.pombe genome deletion project. An update.

Anglický název

Finishing the S.pombe genome deletion project. An update.

Druh

Článek recenzovaný mimo WoS a Scopus

Originální abstrakt

The fission yeast Schizosaccharomyces pombe is a model organism used widely to study various aspects of eukaryotic biology. A collection of heterozygous diploid strains containing individual deletions in nearly all S. pombe genes has been created using a PCR based strategy. However, deletion of some genes has not been possible using this methodology. Here we use an efficient knockout strategy based on plasmids that contain large regions homologous to the target gene to delete an additional 29 genes. the collection of deletion mutants now covers 99% of the fission yeast open reading frames.

Anglický abstrakt

The fission yeast Schizosaccharomyces pombe is a model organism used widely to study various aspects of eukaryotic biology. A collection of heterozygous diploid strains containing individual deletions in nearly all S. pombe genes has been created using a PCR based strategy. However, deletion of some genes has not been possible using this methodology. Here we use an efficient knockout strategy based on plasmids that contain large regions homologous to the target gene to delete an additional 29 genes. the collection of deletion mutants now covers 99% of the fission yeast open reading frames.

Klíčová slova

fission yeast; gene deletion; genomics; essential genes; vectors

Klíčová slova v angličtině

fission yeast; gene deletion; genomics; essential genes; vectors

Autoři

ČARNECKÁ, M.; MÁROVÁ, I.

Rok RIV

2011

Vydáno

28.07.2010

Nakladatel

LANDES BIOSCIENCE

Místo

USA

ISSN

1538-4101

Periodikum

CELL CYCLE

Svazek

9

Číslo

12

Stát

Spojené státy americké

Strany od

2399

Strany do

2402

Strany počet

4

BibTex

@article{BUT50225,
  author="Martina {Čarnecká} and Ivana {Márová}",
  title="Finishing the S.pombe genome deletion project. An update.",
  journal="CELL CYCLE",
  year="2010",
  volume="9",
  number="12",
  pages="2399--2402",
  issn="1538-4101"
}